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全球LC-MS/MS目标代谢组分使用HILIC和RPLC分离模式的组合在基于1-乙烯-1,2,4-三的有机单体柱上

Nikita V Basov¹, Artem D Rogachev¹, Maria A Aleshkova¹

¹N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Acad. Lavrentiev Ave., 9, 630090, Novosibirsk, Russia; Novosibirsk State University, Pirogov Str., 2, 630090, Novosibirsk, Russia.

|September 14, 2023

概括

这项研究引入了一种新的LC-MS/MS方法,使用合成的单立柱进行广泛的代谢物查. 该方法在不到30分钟的时间内有效检测到大约400种代谢物,有助于识别辐射暴露标记物.

科学领域:

分析化学分析化学
代谢学代谢学代谢学
生物化学生物化学

背景情况:

代谢物分析需要用于极性和非极性化合物的多功能分离技术.
现有的方法通常在代谢物覆盖和分析时间方面存在局限性.
开发新的染色学材料对于推进代谢学研究至关重要.

研究的目的:

开发和验证一种LC-MS/MS方法,用于针对不同代谢物的查.
合成和利用一种新的单立柱来增强代谢物分离.
在生物样本中应用开发的用于识别辐射暴露生物标志物的方法.

主要方法:

从 styrene,divinylbenzene 和 1-vinyl-1,2,4-triazole 中合成了一个单立柱.
采用液体染色学-并联质谱法 (LC-MS/MS) 的方法.
使用水友互动液态色谱 (HILIC) 和逆相液态色谱 (RP LC) 以一种互补的方式.

主要成果:

使用合成的柱子实现了极性和非极性代谢物的分离.
通过结合HILIC和RPLC模式,在30分钟内检测到大约400种代谢物.
与商用列相比,证明了更广泛的代谢物覆盖范围.
在小鼠血液样本中确定了X射线辐射暴露和器官损伤的潜在生物标志物.
揭示了特定代谢途径的辐射诱导的变化.

结论:

开发的LC-MS/MS方法与合成的单立柱为广泛的代谢查提供了强大而有效的工具.
该方法提供卓越的代谢物覆盖范围,并且有效地识别生物样本中的生物标志物.
这种方法对于了解辐射暴露的代谢后果有价值.

关键词:

双保留色素学双保留色素学液体色谱学液体色谱学液体色谱学代谢学代谢学代谢学一个单一的柱子.协奏式质谱测量质谱测量在X射线辐射照射中.

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