血红蛋白分数HbA2,HbF和HbA0的儿科参考间隔使用高性能液体染色学和毛细血管电泳
Kasper Munch Lauridsen1, Helle Pilgaard Kristiansen2, Anne Winther-Larsen3
1Department of Clinical Biochemistry, Aarhus University Hospital, Palle Juul-Jensens Boulevard 69, 8200 Aarhus N, Denmark; Steno Diabetes Center Aarhus, Aarhus University Hospital, Palle Juul-Jensens Boulevard 11, Entrance A, 8200 Aarhus N, Denmark; Department of Clinical Medicine, Aarhus University, Palle Juul-Jensens Boulevard, 118200 Aarhus N, Denmark.
Clinica chimica acta; international journal of clinical chemistry
|September 14, 2023
概括
这项研究为血红蛋白分数建立了儿科参考间隔,这对于查β-血病载体至关重要. 它提供了使用HPLC和CE方法对1-18岁儿童的血红蛋白A2 (HbA2) 分析的基本数据.
科学领域:
- 临床化学 临床化学
- 血液学 血液学 血液学
- 儿科诊断 儿科诊断 儿科诊断
背景情况:
- β-thalassemia是一种普遍的遗传性疾病,影响全球数百万人.
- 血红蛋白A2 (HbA2) 升高是beta-thalassemia载体的一个关键指标.
- 对血红蛋白部分缺乏确定的儿科参考间隔 (RIs),使查复杂化.
研究的目的:
- 为了确定血红蛋白分数的儿科参考间隔 (RIs).
- 为此目的使用高性能液态色谱 (HPLC) 和毛细管电泳 (CE).
- 为了解决儿科RI对于HbA2分析的稀缺性.
主要方法:
- 从1-18岁的健康儿童和青少年收集样本.
- 使用Tosoh G11 (HPLC) 和Capillarys 3 Octa (CE) 系统分析的样本.
- 评估了对数据按年龄和性别分区的需求.
主要成果:
- 已确定HbA2的95%RI为2.00-2.90% (HPLC) 和2.2-3.0% (CE). 在此情况下,HbA2的95%RI为2.00-2.90% (HPLC) 和2.2-3.0% (CE).
- 没有发现HPLC的临床相关数据,分别按年龄或性别划分.
- CE数据表明,需要根据年龄进行分区.
结论:
- 在1-18岁的个体中报告了血红蛋白部分的儿科RI.
- 这项研究是第一个使用Tosoh G11 HPLC系统报告儿科HbA2 RI的研究.
- 提供了为儿科β-thalassemia查提供关键参考数据.
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