含有38的卷曲-卷曲域对于小鼠的青体生物发生和纤维组装是必需的
Yaling Wang1, Xueying Huang2, Guoying Sun3
1Shanghai Key Laboratory of Metabolic Remodeling and Health, Institute of Metabolism and Integrative Biology, Fudan University, Shanghai 200438, China; Institute of Reproduction and Development, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
Journal of genetics and genomics = Yi chuan xue bao
|September 14, 2023
概括
在小鼠中,含有38 (Ccdc38) 的卷状卷状域的淘汰会导致由于精子鞭毛缺陷和体缺血等原因导致男性不孕. 这项研究揭示了CCDC38
科学领域:
- 生殖生物学 生殖生物学
- 精子发生是精子生成.
- 男人不孕不育的情况
背景情况:
- 精子生成涉及到受精的关键形态变化,包括精子鞭毛和体形成.
- 旗多重形态异常 (MMAF) 是一种与旗缺陷相关的男性不孕症综合征.
- 青体缺血症可能会损害胚胎发育,但其与MMAF的关联尚未得到充分证实.
研究的目的:
- 为了研究含有38 (Ccdc38) 的卷轴-卷轴域在精子发育和男性生育能力中的作用.
- 为了确定Ccdc38缺陷是否会导致MMAF表型的背景下,在MMAF表型的背景下导致宏体缺血.
- 探索Ccdc38相关不孕症的潜在机制.
主要方法:
- 使用基编辑诱导无意义突变的Ccdc38淘汰赛 (Ccdc38-KO) 鼠标的生成.
- 对Ccdc38-KO精子的表型分析,包括对鞭毛和体的形态评估.
- 生物化学分析以评估TEKT3蛋白水平和丸和精子中的分布.
主要成果:
- Ccdc38-KO雄性小鼠表现出典型的MMAF表型和体缺血的不孕症.
- 精子Ccdc38-KO显示松散定的体膜和无组织的纤维.
- Ccdc38淘汰导致TEKT3水平降低,并在丸和精子中产生异常的TEKT3分布.
结论:
- CCDC38在体生物发生过程中起着至关重要的,以前未被认可的作用.
- 这项研究提供了新的证据,将状体低成形与MMAF联系起来.
- 内细胞质精子注射可以克服Ccdc38相关的不孕症,这表明一种潜在的治疗方法.
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