综合性转录基因分析揭示了番茄植物对Ralstonia solanacearum反应的lncRNA-miRNA-mRNA相互作用
Xiuyang Si1, Hongyan Liu1, Xi Cheng1
1Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
International journal of biological macromolecules
|September 14, 2023
概括
这项研究揭示了非编码RNAs,包括长非编码RNAs (lncRNAs) 和microRNAs (miRNAs) 如何调节番茄对Ralstonia solanacearum细菌枯的耐药性. 确定了关键的监管网络,为疾病抵抗机制提供了新的见解.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 生物化学 生物化学
背景情况:
- 拉尔斯托尼亚·索拉纳西乌姆 (Ralstonia solanacearum) 导致西红显著的细菌枯,影响全球生产.
- 在番茄对细菌枯的反应中,非编码RNA的作用尚不清楚.
- 了解这些调节机制对于开发抗病番茄品种至关重要.
研究的目的:
- 在R. solanacearum感染后全面分析番茄耐药和易受性线的转录格局.
- 为了识别差异表达的非编码RNAs (lncRNAs,miRNAs) 和参与细菌枯抵抗的mRNAs.
- 阐明非编码RNA,包括ceRNA网络和内源性标模仿物 (eTMs) 在番茄-病原体相互作用中的调节作用.
主要方法:
- 使用高通量RNA测序,在耐药 ('ZRS_7') 和易受 ('HTY_9') 番茄系中分析了mRNA和非编码RNA.
- 进行了差异表达分析,基因本体学 (GO) 和KEGG通路分析.
- 竞争的内源RNA (ceRNA) 网络结构和病毒诱导基因沉默 (VIGS) 技术被用来验证监管相互作用.
主要成果:
- 在注射后,在耐药和易受性线之间观察到数千个mRNA,lncRNA和miRNA的显著变化.
- 基因型特异性反应表明,非编码RNA在疾病耐药性方面具有不同的调节作用.
- 构建了一个复杂的ceRNA网络,涉及miRNAs,lncRNAs和mRNAs,突出了像受体类激酶和NBS-LRRs这样的潜在目标.
- 静止一种内源性标模仿物 (eTM482e-3p-1) 显著增加了番茄的敏感性,证实了它在eTM482e-3p-1-Sly-miR482e-3p-NBS-LRRs调节途径中的作用.
结论:
- 非编码RNAs,特别是lncRNAs和miRNAs,在调节番茄对R. solanacearum的反应中起着至关重要的作用.
- 已识别的ceRNA网络和eTM为细菌枯抵抗的基础分子机制提供了新的见解.
- 这些发现为开发新策略铺平了道路,通过操纵非编码RNA通路来增强番茄病耐药性.
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