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酸乙醇被LC-MS/MS检测为酒精消费的潜在新标志物

Matthias Bantle^1,2, Lanya van Tieghem³, Wolfgang Weinmann¹

¹Institute of Forensic Medicine, Forensic Toxicology and Chemistry, University of Bern, Bern, Switzerland.

European journal of mass spectrometry (Chichester, England)

|September 14, 2023

概括

这项研究引入了LysoPEths,这是一种与脂乙醇 (PEths) 结构相关的新型酒精生物标志物. 在人类血液中检测到LysoPEth 16:0,与PEth水平相关,并显示出监测酒精消费的潜力.

科学领域:

分析化学分析化学
临床化学临床化学
法医毒理学法医毒理学

背景情况:

类乙醇 (PEths) 是已确立的直接酒精生物标志物,反映了最近或长期的酒精消费.
量化PEths提供了对个体饮酒行为的洞察力,这对于临床和法医应用至关重要.

研究的目的:

介绍和描述一种新型的酸乙醇物种子类,称为LysoPEths.
开发和验证一种LC-MS/MS方法,用于量化LysoPEth物种和相关的PEths.
在各种条件下研究LysoPEths与PEths的形成和稳定性.

主要方法:

使用酶方法 (PLD,PLA2) 和LC-MS/MS的LysoPEth 16:0的合成和表征.
对多种LysoPEth和PEth物种在MRM模式下开发LC-MS/MS方法.
用乙醇化血液以研究LysoPEth形成和分析临床/法医样本.

主要成果:

确定了一种新的LysoPEths类,结构上与PEths相关,但缺乏脂肪酸链.
成功开发了一种LC-MS/MS方法,用于量化LysoPEth 16:0和其他LysoPEth/PEth物种.
实验室观察到LysoPEth 16:0的形成与不断增加的乙醇度,度大约是PEths的三倍.
在临床和法医样本中检测到LysoPEth 16:0,与PEth 16:0/18:1 (r2 = 0.75) 呈现线性相关性.

结论:

莱索PEths代表了一组新的直接酒精生物标志物,具有潜在的临床和法医实用性.
开发的LC-MS/MS方法可靠地检测和量化LysoPEths与PETths一起.
lysopeth度与PEth水平相关,这表明它们在评估酒精消费模式中的有用性.

关键词:

这是LC-MS/MS.这就是LysoPEth.酒精生物标志物酒精生物标志物血液,干燥的血液斑点血.开发方法开发方法的方法.酸乙醇 (PET) 是一种酸.

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