一个新的可切换的水静止阶段用于超临界流体染色学
Emmanuel A Nai1, Kevin B Thurbide1
1Department of Chemistry, University of Calgary, 2500 University Drive, NW, Calgary, Alberta, T2N 1N4, Canada.
Analytica chimica acta
|September 14, 2023
概括
超临界流体染色学 (SFC) 的新型pH可切换水静止相允许精确控制分析物保留. 这种方法可以在酸性和性条件之间快速切换,显著提高分离选择性和分辨率.
科学领域:
- 分析化学 分析化学
- 染色体学 染色体学 是一种染色学.
背景情况:
- 超临界流体染色学 (SFC) 是一种强大的分离技术.
- 控制静止相性质对于优化色谱分离至关重要.
研究的目的:
- 为SFC开发和描述一种新的pH可切换水静止相.
- 通过改变静止阶段pH值来证明操纵分析物保留和选择性的能力.
主要方法:
- 一个水静止阶段被涂覆并用氧化 (NH 4 OH) 水合.
- 超临界流体染色学 (SFC) 使用二氧化碳 (CO2) 作为移动相.
- 静止阶段的pH值通过调整CO2压力,温度和NH4OH度来调节.
- 在不同的pH条件下监测了分析物的保留和化行为.
主要成果:
- 通过操纵CO 2的压力和温度,可以实现水静止阶段的广泛pH范围 (大约3-9).
- 在酸性和基本性静止相条件之间快速切换 (在18秒内) 改变了分析物的保留性质.
- 对于可电离分析剂,观察到选择性 (高达35倍) 和分辨率 (高达40倍) 的显著改善.
- 在pH值切换后保留时间的可复制性很高 (1.4% RSD).
结论:
- 新的pH可切换水静止阶段为SFC中的染色分离提供了动态控制.
- 这种技术为可电离分析剂提供了增强的选择性和分辨率.
- 该系统通过改进目标分析物和矩阵组分差异化来证明复杂混合物分离的潜力.
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