基于源微滴驱动衍生物的自由脂肪酸的快速和灵敏的确定,加上高分辨率质谱仪
Jiaqi Cui1, Jianxun Zhang2, Shihao Sun2
1Flavor Research Center, Zhengzhou University, Zhengzhou, 450001, China.
Analytica chimica acta
|September 14, 2023
概括
一种新方法使用微滴衍生和质谱来对生物样本中敏感的自由脂肪酸 (FFA) 分析. 这种技术可靠地检测出12个FFA,有助于疾病诊断和研究.
科学领域:
- 分析化学 分析化学
- 生物化学 生化学
- 环境科学 环境科学
背景情况:
- 准确测量自由脂肪酸 (FFAs) 对于疾病诊断和预后至关重要.
- 现有的FFA分析方法可能缺乏灵敏度或需要复杂的样本准备.
研究的目的:
- 开发一种敏感且直接的方法,用于在生物样本中分析FFA.
- 建立源微滴衍生策略与高分辨率质谱相结合,用于FFA量化.
主要方法:
- 在源中使用2-皮可胺 (PA) 通过电喷剂进行微滴衍生.
- 高分辨率质谱仪用于直接分析衍生型FFA.
- 对12个常见FFA的方法的验证,包括对线性,LOD和LOQ的评估.
主要成果:
- 可靠地确定了12个具有高灵敏度的典型FFAs (LODs 9-76 pg mL-1).
- 对于分析的FFA,可以达到可接受的线性 (R2 ≥0.94).
- 该方法在PFOS暴露后成功分析了大鼠肝脏和脏样本中的FFA变化.
结论:
- 开发的源微滴衍生和质谱法为FFA分析提供了可靠的方法.
- 该技术在复杂的生物矩阵中检测FFAs方面显示出有前途的应用.
- 这种方法可以帮助理解FFA在疾病发病和环境暴露研究中的作用.
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