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促进ABCG2基因表达由来自Piper longum L L的新植物

Atsuyoshi Nishina¹, Motohiko Ukiya², Kazuki Motegi²

¹School of Health and Nutrition, Tokai Gakuen University, Nagoya, Aichi, Japan.

Bioscience, biotechnology, and biochemistry

|September 14, 2023

概括

研究人员从Piper longum L.中发现了促进ABCG2基因表达的化合物. 利卡林A和新烯显示出通过AhR通路增强ABCG2表达的潜力.

科学领域:

药理学药理学是指药理学的学科.
自然产品化学化学
分子生物学分子生物学

背景情况:

长 (Piper longum L.) 是缅甸的传统草药,具有已知的保护性.
ABCG2基因在异生菌的运输和细胞保护中起着至关重要的作用.
识别调节ABCG2表达的自然化合物对于治疗开发很重要.

研究的目的:

从Piper longum L.中分离和识别增强ABCG2基因表达的化合物.
评估这些化合物作为阿里碳化合物受体 (AhR) 途径的激动剂的潜力.

主要方法:

用甲醇提取Piper longum L. (不包括水果) 的上空部分.
使用色谱和光谱技术分离和识别六种化合物.
使用实时PCR对Caco-2细胞中 hABCG2mRNA表达的评估.
评估化合物的毒性和通过siRNA介导的hAhR.击倒作用机制.

主要成果:

从P. longum甲醇提取物中分离出六种化合物.
利卡林A和新烯在Caco-2细胞中表现出显著的HABCG2mRNA表达增强效应,毒性低.
通过siRNA介导的hAhR抑制降低了利卡林A和新胺的活性,类似于已知的AhR激动剂3-甲基胆烯 (3MC).

结论:

利卡林A和新胺被确定为Piper longum L.中的生物活性化合物,具有增强 hABCG2基因表达的能力.
这些化合物可能作为hAhR激动剂起作用,这表明调节ABCG2表达的新机制.
这些发现支持Piper longum L.衍生物在涉及ABCG2调节的条件下潜在的治疗应用.

关键词:

长长L L长L L长L长这就是ABCG2ABCG2.这是一个对接模拟模拟.一只山羊,一只山羊.这就是新教派 (neolignans).

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