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在产前唐氏综合征的查中循环微RNAs

Senay Balci¹, Filiz Cayan², Gurbet Dogru Ozdemir³

¹Assoc. Prof., Mersin University Medical Faculty, Department of Medical Biochemistry, Mersin, Turkey.

Puerto Rico health sciences journal

|September 14, 2023

概括

这项研究研究了微RNAs (miRNAs) 作为胎儿出生缺陷的非侵入性产前诊断的生物标志物. 母体血中特定miRNA的水平升高可能表明染色体异常,提供了一个有前途的诊断工具.

科学领域:

遗传学遗传学是一个
分子生物学分子生物学
产科产科产科产科产科

背景情况:

产前查遗传缺陷和染色体形瘤是至关重要的.
目前的方法包括超声波,母体血清查,以及诸如乳液切割等具有风险的侵入性手术.
使用循环的胎儿核酸进行非侵入性产前诊断是一个新兴的领域.

研究的目的:

探索微RNAs (miRNAs) 作为非侵入性产前诊断的生物标志物的潜力.
研究母体血和羊水中的miRNA表达特征,以检测胎儿异常.

主要方法:

使用定量实时聚合酶连锁反应 (qPCR) 来分析miRNA表达.
从孕妇患有胎儿先天性异常 (患者组) 和没有异常的对照组收集样本.
分析了母亲的血和羊水样本.

主要成果:

特定的miRNAs (hsa-miR-629-5p,hsa-miR-320c,hsa-miR-21-5p,hsa-let-7c-5p,hsa-miR-98-5p,hsa-miR-486-5p,hsa-miR-4732-5p和hsa-miR-181a-5p) 在患者组的母亲血中显示出水平的增加.
这些升高的miRNA水平与对照组的水平进行了比较.

结论:

微RNA显示出潜在的价值生物标志物,用于胎儿出生缺陷的非侵入性产前诊断.
进一步的研究可能会确定miRNAs作为关键指标,特别是在像唐氏综合征这样的疾病中.

关键词:

这是一种先天性异常.唐氏综合征是什么意思唐氏综合征微RNAs是一种微RNA.怀孕怀孕怀孕怀孕产前诊断在产前诊断.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...