Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A special multifiber dietary mixture ameliorates Crohn's-like colitis in an IL-10<sup>-</sup>/<sup>-</sup> mouse model by promoting treg differentiation through the ETS1/RUNX1/Foxp3 axis.

European journal of nutrition·2026
Same author

Targeting rapidly cycling receptors CD2 and CD7 increases nanoparticle delivery to primary CD4<sup>+</sup> T cells.

Nature communications·2026
Same author

Real-World Outcomes of Barbed Versus Interrupted Sutures for Laparoscopic Choledochotomy: A 12-Month Follow-Up in Patients With Choledocholithiasis.

Surgical laparoscopy, endoscopy & percutaneous techniques·2026
Same author

Entry Without Expression: Internalisation Does Not Predict mRNA Translation for Targeted Lipid Nanoparticles.

Small science·2026
Same author

Introduction to "Endocytosis and cellular delivery".

RSC chemical biology·2026
Same author

Hybrid macrophage-mitochondria extracellular vesicles for mitochondrial ROS regulation in diabetic wounds.

Nature communications·2026

相关实验视频

Updated: Jul 16, 2025

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning
12:07

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Published on: April 16, 2018

13.5K

自燃聚合物纳米粒子具有精确和可控制的pH依赖性降解.

Samuel A Smith1, Bruna Rossi Herling2, Changhe Zhang1

  • 1Department of Chemistry, The University of Melbourne, Parkville, Victoria 3010, Australia.

Biomacromolecules
|September 14, 2023
PubMed
概括

新的自燃聚合物纳米粒子提供可调节的pH触发药物释放. 4:1的PGAm纳米颗粒显示出细胞内传递的希望,证明了高效的内体体逃生和受控的降解.

更多相关视频

Nanosponge Tunability in Size and Crosslinking Density
11:15

Nanosponge Tunability in Size and Crosslinking Density

Published on: August 4, 2017

7.7K
Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

7.5K

相关实验视频

Last Updated: Jul 16, 2025

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning
12:07

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Published on: April 16, 2018

13.5K
Nanosponge Tunability in Size and Crosslinking Density
11:15

Nanosponge Tunability in Size and Crosslinking Density

Published on: August 4, 2017

7.7K
Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

7.5K

科学领域:

  • 材料科学 材料科学 材料科学
  • 纳米技术 纳米技术
  • 生物医学工程 生物医学工程

背景情况:

  • 聚合物纳米粒子在治疗性货物交付方面得到了广泛的研究.
  • 自焚聚合物 (SIP) 能够控制分解和货物释放.
  • 响应pH的材料对于向的细胞内药物输送至关重要.

研究的目的:

  • 开发适应pH的自燃聚合物纳米颗粒,用于药物输送.
  • 通过改变聚合物组成来调整纳米颗粒的分解pH值.
  • 评估开发的纳米颗粒的载荷,降解和内体体逃脱效率.

主要方法:

  • 纳米颗粒的合成使用聚乙烯糖醇 (poly) -b-2-diisopropyl (amino ethyl methacrylate) 和一种自燃聚合物P (DPAEGAm-r-DBAEGAm).
  • 在P ((DPAEGAm-r-DBAEGAm) 中变化了二烯胺与二烯胺替代物的比例,以创建四种类型的PGAm颗粒 (4:1, 2:1, 2:3, 0:1).
  • 评估了粒子分解pH值,不同pH值的聚合物脱聚合率,载荷载量和内体逃生效率.

主要成果:

  • 拆解pH值可以通过调整氨基替代物比率从7.0调整到5.0.
  • 在2小时内,P(DPAEGAm-r-DBAEGAm) 聚合物表现出明显的脱聚合 (60-80%) 低于分解pH值,pH值为7.4.4时降解最小 (<10%).
  • 4:1 PGAm纳米颗粒与其他配方 (<1%) 相比,表现出更高的内体逃生效率 (∼4%),而所有加载的载荷.

结论:

  • 开发的PGAm纳米粒子提供可调节的,pH触发的降解,用于控制的货物释放.
  • 4:1的PGAm纳米粒子配方显示出作为有效的细胞内药物递送系统的潜力.
  • 4: 1 PGAm纳米粒子的进一步优化对于先进的治疗应用是有必要的.