一种转录因子ZmGLK36在谷物作物中赋予了对玉米粗矮病的广泛抵抗力
Zhennan Xu1, Zhiqiang Zhou1, Zixiang Cheng1
1State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Nature plants
|September 14, 2023
概括
研究人员将ZmGLK36确定为一种关键基因,该基因赋予玉米中对大米黑条纹矮人病毒 (RBSDV) 的耐药性. 这种基因通过促进莉酸 (JA) 生产来增强植物防御能力,为作物育种提供了新的策略.
科学领域:
- 植物病理学 植物病理学
- 分子遗传学 分子遗传学
- 农作物科学 农作物科学
背景情况:
- 玉米粗矮病 (MRDD) 是由像RBSDV这样的病毒引起的,对全球谷物生产构成重大威胁.
- 开发耐药作物品种对于粮食安全至关重要.
研究的目的:
- 精确地绘制和克隆玉米中RBSDV耐药性的定量特征位置 (QTL).
- 阐明已识别的QTL所赋予的RBSDV耐药性的基础分子机制.
主要方法:
- 在玉米中的qMrdd2位点的精细映射和基因克隆.
- 分析ZmGLK36基因功能与莉酸 (JA) 生物合成和防御途径的关系.
- 在ZmGLK36 5' UTR.中识别和描述一个26-bp indel.
- 研究ZmDBF2与ZmGLK36促进体之间的相互作用.
- 在大米和小麦中采用转基因和标记辅助方法来评估保存的耐药性.
主要成果:
- qMrdd2位点被确定为ZmGLK36,一种类似G2的转录因子.
- ZmGLK36通过促进JA生物合成和JA介导的防御来增强RBSDV的耐药性.
- 在ZmGLK36 5' UTR中的26-bp内与差异性基因表达和RBSDV耐药性有关.
- 一个AP2/EREBP转录因子ZmDBF2直接结合了indel并抑制了ZmGLK36的表达.
- 在大米和小麦中,ZmGLK36赋予了对RBSDV的保护性耐药性.
结论:
- ZmGLK36是玉米中RBSDV抗性的关键调节剂,通过JA介导的途径起作用.
- 在ZmGLK36的5' UTR中有一种特定的印位会影响耐药性,为繁殖提供潜在的标志物.
- 了解ZmGLK36-ZmDBF2相互作用,可以了解病毒防御机制.
- ZmGLK36的保护作用凸显了它在全球培育耐药谷物作物的潜力.
相关概念视频
02:22Introduction to Plant Diversity
From Water to Land
02:50Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...