人类内源病毒K6和K11的插入变异在Normozoospermic男性中
Neslihan Hekim1, Sezgin Gunes2,3, Sercan Ergun2,3
1Faculty of Medicine, Department of Medical Biology, Ondokuz Mayis University, Samsun, 55139, Turkey. neslihan.taskurt@omu.edu.tr.
Reproductive sciences (Thousand Oaks, Calif.)
|September 14, 2023
概括
人体内源逆转录病毒K6 (HERV-K6) 和K11 (HERV-K11) 插入可能与男性不孕症有关. 这项研究发现,不孕不育和生育不育的男性之间的HERV-K6和HERV-K11插入率存在差异.
科学领域:
- 生殖生物学 生殖生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 男性不孕症是一种复杂的疾病,受遗传,表观遗传和环境因素的影响.
- 逆转移子的改变表达,如人类内源逆转录病毒 (HERVs),已与精子生成受损有关.
研究的目的:
- 研究HERV-K6和HERV-K11插入变异在normozoospermic男性中异常男性不孕症的潜在作用.
- 分析不育和肥沃的诺莫动物精子个体之间的HERV-K6和HERV-K11插入率的差异.
主要方法:
- 基因组DNA从41名不育和45名肥沃的诺莫动物精子男性的血液样本中分离出来.
- 使用互逆转移素多态聚合酶链反应 (IRP-PCR) 来分析HERV-K6和HERV-K11插入变异.
主要成果:
- 在不孕群中,HERV-K6的变异率为0-58.3%,在生育群中为0-53.4%.
- 在不孕群中,HERV-K11的变异率为0-75.0%,在生育群中为0-77.8%.
- 与不育群相比,在生育群中观察到HERV-K6和HERV-K11插入率显著更高 (分别P < 0.0001和P = 0.007).
结论:
- 在HERV-K6和HERV-K11逆转移素插入中,个体间存在差异.
- 这些插入变异可能与男性不孕症的发展有关.
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