生理学,代谢学和转录学揭示了Gymnocypris przewalskii由于暴露于寒冷而导致的代谢途径变化
Sijia Liu1, Fei Tian1, Delin Qi2
1Qinghai Provincial Key Laboratory of Animal Ecological Genomics, Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No. 23 Xinning Road, Xining, 810008, Qinghai, China.
BMC genomics
|September 14, 2023
概括
西藏高原鱼类利用替代拼接和激活的抗氧化过程来适应冷水. 这项研究揭示了能量代谢中的关键基因-代谢物相互作用,用于耐寒性.
科学领域:
- 远鱼的环境适应 远鱼的环境适应
- 耐寒性的分子机制
- 高海拔的水生生态系统
背景情况:
- 鱼表现出寒冷的适应性,但西藏高原物种的机制尚不清楚.
- Gymnocypris przewalskii是西藏高原的特有生物,面对寒冷的环境.
- 了解寒冷适应对于保护高海拔水生生物至关重要.
研究的目的:
- 研究Gymnocypris przewalskii对寒冷条件的生理和分子反应.
- 为了确定关键的基因,代谢物和涉及寒冷适应的途径.
- 为了阐明西藏高原鱼类的寒冷耐受机制.
主要方法:
- 来自适应寒冷 (4°C) 和正常 (17°C) 温度的鱼的八种组织的转录组测序 (RNA-seq).
- 液体染色学-质谱学 (LC-MS/MS) 肠道,肝和肌肉的代谢学分析.
- 生物信息分析包括差异基因表达,基因本体学和基因和基因组路径分析的京都百科全书.
主要成果:
- 鉴定了5,745个差异表达基因 (DEGs),这些基因在结合体通路中丰富,表明了活跃的替代拼接.
- 分别在肠道,肝和肌肉中发现了82,97和66种差异表达的代谢物.
- 谷氨代谢是重叠的关键途径,表明激活了抗氧化反应.
结论:
- 替代拼接和激活的抗氧化过程对于Gymnocypris przewalskii来说至关重要,以应对寒冷压力.
- 能量代谢途径,包括葡萄糖生成和长链脂肪酸代谢,在维持葡萄糖平衡和在寒冷条件下提供能量方面发挥着至关重要的作用.
- 一个全面的基因代谢物相互作用网络突出显示了这种独特物种对寒冷的综合生理反应.
相关概念视频
Responses to Heat and Cold Stress
13.6K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.6K
Adaptations that Reduce Water Loss
25.7K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
25.7K
Responses to Salt Stress
13.2K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
13.2K
Requirements for Human Life
8.3K
The Earth and its atmosphere have provided humans with air, water, and food, but these are not the only requirements for survival. Humans also require a specific range of temperature and pressure that the Earth and its atmosphere provides.
Oxygen
Atmospheric air is only about 20 percent oxygen, but that oxygen is a key component of the chemical reactions that keep the body alive, including the reactions that produce ATP. Brain cells are susceptible to a lack of oxygen because they require a...
Oxygen
Atmospheric air is only about 20 percent oxygen, but that oxygen is a key component of the chemical reactions that keep the body alive, including the reactions that produce ATP. Brain cells are susceptible to a lack of oxygen because they require a...
8.3K
Background and Environment Affect Phenotype
6.6K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.6K
Decreased Body Temperature
643
A decreased body temperature can occur in patients with hypothermia and frostbite. Heat loss with extended cold exposure overpowers the body's ability to create heat, resulting in hypothermia. Core temperature readings help classify hypothermia. Mild hypothermia is temperatures between 32 °C (89.6 °F) and 35°C (95 °F) and is caused by impaired thermoregulation. Moderate hypothermia is temperatures between 28 C (82.4 °F) and 32 °C (89.6 °F) caused by...
643


