发现与鼻癌中基因表达相关的ceRNA网络和DNA甲基化
Ting Zhang1, Lu Pei2, Wen-Li Qiu2
1Center of Reproductive medicine, Affiliated hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.
BMC medical genomics
|September 14, 2023
概括
这项研究揭示了竞争性内源性RNA (ceRNA) 网络和DNA甲基化如何影响鼻癌 (NPC) 的进展. 在lncRNA RP11-545G3.1促进NPC细胞活力,迁移和入侵.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 鼻癌 (NPC) 是一个重要的健康问题,具有复杂的分子基础.
- 了解基因表达调节之间的相互作用,例如竞争性内源性RNA (ceRNA) 网络,以及像DNA甲基化这样的表观遗传修饰,对于破译NPC病变产生至关重要.
研究的目的:
- 识别由ceRNA网络和NPC中的DNA甲基化调节的异常表达基因.
- 构建一个ceRNA网络并研究这个网络内的长非编码RNAs (lncRNAs) 在NPC进展中的作用.
- 为了验证特定lncRNAs在NPC细胞行为中的功能意义.
主要方法:
- 使用公开数据集 (GSE64634,GSE32960,GSE95166,GSE126683,GSE62336) 来选NPC中差异表达的mRNA,miRNA和lncRNA.
- 构建了一个ceRNA网络,并确定了受ceRNA活动和DNA甲基化影响的基因.
- 使用RT-qPCR验证了NPC组织和细胞中lncRNA RP11-545G3.1的表达,并通过敲击实验评估了其功能影响.
主要成果:
- 建立了一个包含3个lncRNA,15个miRNA和129个mRNA的ceRNA网络.
- 确定了由ceRNA机制和DNA甲基化调节的基因,包括HMGA1与患者存活的关联.
- 发现RP11-545G3.1在NPC中高度表达,并证明其敲除显著降低了NPC细胞活力,迁移和入侵.
结论:
- 表观遗传调节,特别是通过ceRNA网络和DNA甲基化,在鼻癌中起着关键作用.
- lncRNA RP11-545G3.1被认为是NPC进展的关键调节者,影响瘤细胞活力和转移.
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