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转录因子c-Maf驱动巨细胞促进过度缩的痕形成

Liu Yang¹, Yajuan Song¹, Tong Wang¹

¹Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

Journal of cosmetic dermatology

|September 15, 2023

概括

c-Maf阳性M2巨细胞通过TGF-β1.1.的分泌增加纤维细胞增殖,迁移和细胞外基质沉积,从而促进过度缩性痕的形成.

科学领域:

伤口愈合研究研究研究.
细胞生物学细胞生物学
免疫学免疫学免疫学

背景情况:

过度缩性痕 (HS) 是由纤维细胞过度生长和过度细胞外基质 (ECM) 沉积引起的.
在繁殖阶段,c-Maf阳性M2巨细胞在HS组织中普遍存在.
在HS中调节纤维细胞行为的c-Maf阳性M2巨细胞的作用尚不清楚.

研究的目的:

为了研究c-Maf阳性M2巨对缩性痕纤维细胞 (HFB) 生物行为的影响.
阐明c-Maf阳性M2巨细胞影响HS形成的潜在机制.

主要方法:

人类HS和正常皮肤 (NS) 的组织学分析 (HE,马森三色素).
免疫光染色用于识别和量化c-Maf+/CD68+ M2巨细胞.
在体外测试 (CCK8,伤口愈合,Transwell) 以评估纤维细胞的增殖和迁移.
西方斑块和qPCR用于分析基因和蛋白质表达 (COL1,COL3,α-SMA,TGF-β1).

主要成果:

与NS相比,HS中观察到c-Maf+/CD68+ M2巨细胞的增加.
与c-Maf过度表达的M2巨细胞或它们的浮体共同培养增强了HFB和正常皮肤纤维细胞 (NFB) 的增殖和迁移.
在暴露于c-Maf过度表达的M2巨细胞或它们的超体的纤维细胞中,观察到COL1,COL3和α-SMA的表达升高.
过度表达c-Maf的M2巨体表现出增加的TGF-β1表达.

结论:

c-Maf阳性巨细胞有助于缩性痕的形成.
这些巨细胞调节纤维细胞的增殖,迁移和ECM沉积.
在这个过程中,c-Maf阳性巨细胞的TGF-β1分泌是关键机制.

关键词:

c-Mafaf 的意思是纤维细胞细胞是什么?过度缩的痕过度缩的痕巨细胞是一个巨细胞.

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