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Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

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宽带图解使用曲线波照明.

Daniel S Penagos Molina, Lars Loetgering, Wilhelm Eschen

|September 15, 2023

概括

在光学学中定制照明曲率可以实现更广泛的光谱带宽,从而实现更高的流量和吞吐量. 这一进步缓解了时间连贯性需求,有利于同步机和实验室尺度成像.

科学领域:

一致的成像成像.
衍射成像技术是不同的成像技术.

背景情况:

图形学依赖于连贯的照明,限制光谱带宽.
时间连贯效应通常限制了在衍射成像中可用的光谱范围.

研究的目的:

为了研究光谱带宽和照明曲率在光谱学中的关系.
探索在无需复杂的算法变更的情况下利用更广泛的光谱带宽在图解学中的方法.

主要方法:

分析定制照明差异对光谱带宽宽容量的影响.
调查波面曲率如何改变有效衍射几何形状.
检查曲率对时间连贯性要求的影响.

主要成果:

通过量身定制照明分歧,可以容忍更广泛的光谱带宽.
强大的波面曲率将几何转向近场状态,减少对时间连贯性的敏感性.
宽松的连贯性要求允许更广泛的光谱带宽和更大的照明点.

结论:

照明曲率是管理光谱带宽的关键参数.
这项工作使粉红色和宽带光束的使用成为可能,增加了流量和吞吐量.
这些发现适用于同步仪和实验室尺度的图像学光束线.

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