基于光纤组件的高速TIRF和2D超分辨率结构化照明显微镜具有很大的视野
Optics express
|September 15, 2023
概括
我们开发了一种紧的,具有成本效益的超高分辨率结构化照明显微镜 (SR-SIM),可以实现高速的多色成像. 这种新的基于纤维的SR-SIM系统可视化了细胞结构,如纳米分辨率的窗体.
科学领域:
- 生物物理学的生物物理.
- 光学显微镜的使用方法
- 细胞生物学 细胞生物学
背景情况:
- 超分辨率结构化照明显微镜 (SR-SIM) 提供了高灵活性,速度和最小的干扰,超过了光学衍射极限.
- 现有的SR-SIM仪器通常是昂贵的,重的,视野和成像速度有限的.
- 需要更容易获得的,高性能的SR-SIM系统,用于先进的生物成像.
研究的目的:
- 开发新一代紧,经济高效,高速的SR-SIM仪器.
- 集成基于光纤的光学来增强多色二维和全内部反射光SIM (TIRF-SIM) 成像.
- 展示开发的系统对细胞纳米结构的高分辨率可视化能力.
主要方法:
- 光学,电子和软件的紧密集成为一种基于光纤的SR-SIM系统.
- 实现多颜色的2D-SR-SIM和TIRF-SIM成像功能.
- 利用该系统对大鼠肝脏中阴侧内皮细胞中的细胞内结构进行成像.
主要成果:
- 实现了具有大视野 (150×150μm2) 和高成像速率 (高达44Hz) 的SR-SIM.
- 保持在100nm以下的时空分辨率,超过光学衍射极限.
- 在大鼠肝脏中成功可视化了跨细胞纳米孔 (fenestrations).
结论:
- 开发的紧,经济高效的基于光纤的SR-SIM系统可以实现高速,多色超高分辨率成像.
- 这项技术显著提高了SR-SIM在生物研究中的可访问性和应用范围.
- 该系统提供了前所未有的微细细胞结构的可视化,例如内皮窗体.
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