Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Altitude-dependent variations in environmental conditions and human activities regulate microbial community assembly and carbon metabolism patterns in headwater rivers.

Environmental research·2026
Same author

Four decades of Landsat data reveal the evolution of nitrogen and phosphorus components driven by human activities in the northern South China Sea.

Journal of environmental management·2026
Same author

Activation of SNr GABA neurons drives liver-brain-eye axis dysfunction in hepatic encephalopathy.

iScience·2026
Same author

Two-dimensional nonlinear structured illumination microscopy with rsEGFP2.

Biomedical optics express·2025
Same author

Self-interference digital holography with computational aberration correction.

Optics express·2025
Same author

Two-Dimensional Nonlinear Structured Illumination Microscopy with rsEGFP2.

bioRxiv : the preprint server for biology·2025
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
查看所有相关文章

相关实验视频

Updated: Jul 16, 2025

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.3K

为单分子局部化优化自我干扰数字全息图.

Shaoheng Li, Peter A Kner

    Optics express
    |September 15, 2023
    PubMed
    概括
    此摘要是机器生成的。

    用单分子局部化显微镜 (SMLM) 优化自干扰数字全息 (SIDH) 提高了纳米精度成像. 缩小全息图的大小提高了性能,使得微弱的精确定位,不连贯地发射物体在大轴范围.

    更多相关视频

    Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM
    07:27

    Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM

    Published on: November 1, 2017

    10.4K
    Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
    00:10

    Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

    Published on: September 5, 2019

    8.3K

    相关实验视频

    Last Updated: Jul 16, 2025

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

    Published on: February 8, 2014

    12.3K
    Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM
    07:27

    Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy DHM

    Published on: November 1, 2017

    10.4K
    Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
    00:10

    Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

    Published on: September 5, 2019

    8.3K

    科学领域:

    • 光学显微镜是一种光学显微镜.
    • 纳米技术纳米技术
    • 生物物理学的生物物理.

    背景情况:

    • 自干扰数字全息 (SIDH) 能够使用二维图像在很大轴距范围内对不连贯的发射物体进行成像.
    • 将SIDH与单分子定位显微镜 (SMLM) 结合起来,可以在没有机械重定位的情况下,在广的轴距范围内提供纳米精度定位.
    • 背景光显著降低SIDH性能,因为巨大的全息图大小.

    研究的目的:

    • 通过研究全息图半径在不同背景光条件下对定位精度的影响,优化自我干扰数字全息 (SIDH) 性能.
    • 为了确定SIDH的最佳全息半径 (Rh),将其与SMLM相结合,以改善不连贯发射物体的成像.

    主要方法:

    • 进行模拟以分析全息半径 (Rh) 对不同背景光子水平的SIDH性能的影响.
    • 基于模拟结果开发并实验验证了一个优化的SIDH系统.
    • 使用光板SIDH来增强点类源的重建.

    主要成果:

    • 模拟表明,减少全息图大小可以提高定位精度,在10微米轴距范围内,在低信号 (6000光子) 和10光子/像素的背景噪声下,横向达到60nm,轴向达到80nm.
    • 实验验证表明,成功检测出发光子最少为2120的点源.
    • 使用光片SIDH实现了在10微米轴距范围内发射4200光子的点状源的重建.

    结论:

    • 在SIDH系统中优化全息图尺寸对于减轻背景噪声和提高定位精度至关重要.
    • 优化的SIDH-SMLM方法使微弱,不连贯地发射生物样本的高精度,广视场的3D成像成为可能.
    • 这种技术提供了一个强大的解决方案,用于纳米尺度成像在扩展轴的范围,没有机械调整.