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集成微流体芯片用于直接从阳性血液培养物中快速进行抗菌感应性测试

Meijia Zhu¹, Teng Xu^2,3, Yongqiang Cheng¹

¹School of Environmental Science and Engineering, Institute of Eco-Environmental Forensics, Shandong University (Qingdao), Qingdao, Shandong 266237, China.

Analytical chemistry

|September 15, 2023

概括

一个新型细菌分离综合抗微生物敏感性测试 (AST) 芯片快速识别血液感染病原体. 这个芯片可以将测试时间从几天加快到几个小时,从而改善患者护理并降低死亡率.

科学领域:

生物技术是生物技术.
医学诊断医学诊断医学诊断
微生物学微生物学

背景情况:

血流感染 (BSI) 需要快速的抗菌药物处方以获得有效的治疗和降低死亡率.
对于BSI,传统的抗微生物敏感性测试 (AST) 是缓慢的,通常需要2-3天,延迟关键的治疗决策.

研究的目的:

开发和验证一种新型细菌分离集成AST (BSI-AST) 芯片,用于快速识别病原体和敏感性测试.
从阳性血液培养 (PBC) 显著减少AST所需的时间.

主要方法:

BSI-AST芯片集成了细菌分离和AST室,用于芯片上的处理.
细菌通过隔离剂凝和芯片离心法迅速从PBC中分离出来.
真空辅助的和离心机辅助的缩使得在几个小时内可以快速评估表型敏感性.

主要成果:

BSI-AST芯片在不到10分钟的时间内实现了细菌提取,在不到3小时的时间内获得了敏感性结果.
使用大肠杆菌 (Escherichia coli) 进行的概念验证测试显示,总过程时间不到3.5小时.
临床验证显示,93.3%的人与标准AST方法完全一致.

结论:

集成的BSI-AST芯片提供了一种可靠且显著更快的血液感染诊断方法.
这项技术在改善临床诊断和指导抗微生物治疗方面具有巨大的潜力.

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