对转录基因和塑体发育的比较分析揭示了果肉中的不同类胡卜素积累
Nitisha Bhargava1,2, Charles Ampomah-Dwamena1, Charlotte Voogd1
1The New Zealand Institute for Plant and Food Research Limited (Plant & Food Research) Mt Albert, Auckland Mail Centre, Auckland, New Zealand.
Frontiers in plant science
|September 15, 2023
概括
果肉的颜色是由胡卜素积累和塑类型决定的. 色和黄色的桃肉体显示了增加的胡卜素合成和晶体结构,与绿色的肉体不同.
科学领域:
- 植物生物学 植物生物学
- 生物化学 生物化学
- 遗传学 是一个遗传学.
背景情况:
- 胡卜素是植物和人类中必不可少的异类,影响植物发育,并提供像维生素A前体这样的健康益处.
- 基维果 (Actinidia spp.) 是一种植物. 品种表现出不同的肉色 (绿色,黄色,色) 由于不同的胡卜素度.
- 了解Actinidia中的胡卜素合成和封存对于通过繁殖提高营养价值至关重要.
研究的目的:
- 在三种Actinidia物种中研究卡洛化物积累和肉色变化的分子机制.
- 为了将基因表达模式与不同颜色的果中的胡卜素形状和塑体形态相关联.
主要方法:
- 色 (OF),黄色 (YF) 和绿色肉质 (GF) 基维的染色体的显微镜分析.
- 超高性能液态染色学 (UPLC) 用于确定胡卜素样式.
- 用RNA测序 (RNA-seq) 来比较OF和GF物种之间的基因表达.
主要成果:
- 胡卜素在YF和OF染色体中以晶体形式积累,在OF中则有较大的晶体.
- 在OF和YF中,β-胡卜素占主导地位,而在GF中,黄蛋白占主导地位.
- 与GF相比,RNA-seq显示OF中上游胡卜素生物合成基因 (例如DXS,PSY) 和叶绿素降解基因 (例如SGR,NYC1) 的表达更高.
- 在OF中观察到路径调节和塑形态基因 (OR,FIBRILLIN) 的升级.
结论:
- 色基维果的表型来自于胡卜素路径的协调变化,包括增强的合成和特定的塑形态.
- 在成熟过程中的降解与色素在色肉中的碳化合物产量增加有关.
- 遗传和形态因素相互作用,以确定actinidia中的胡卜素分离和最终的肉色.
关键词:
通过RNA测序来测序RNA序列.胡卜素是一种胡卜素.叶绿素的含量 叶绿素的含量叶绿体 叶绿体 叶绿体 叶绿体染色体是染色体中的一个.果 (Kiwi) 是一种果.乙胡卜素 (β-胡卜素) 是一种碳酸盐.更多相关视频
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