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化学核溶解与动态负荷相结合,用于诱导牛尾部脊椎间盘的退化

Andrea Vernengo¹, Helen Bumann¹, Nadine Kluser¹

¹AO Research Institute Davos, Davos, Switzerland.

Frontiers in bioengineering and biotechnology

|September 15, 2023

概括

这项研究比较了papain,chondroitinase ABC和collagenase II用于椎间盘 (IVD) 退化模型. 帕帕因和原酶诱导了晚期退化,而冠状腺酶ABC模仿了早期阶段,为研究提供了一种光谱.

科学领域:

生物医学工程生物医学工程
整形外科整形外科整形外科
组织工程是组织工程.

背景情况:

化核溶解是创建椎间盘 (IVD) 退化器官培养模型的关键方法.
需要直接比较主要的化学核分解酶,以了解它们对人类IVD条件的退行性模仿.

研究的目的:

为了比较分析 papain,chondroitinase ABC (chABC) 和 collagenase II (col'ase) 对牛骨内脏器的退行性影响.
评估这些酶诱导退化模型如何反映人类IVD退化的不同阶段.

主要方法:

牛尾体IVD经过使用特定度的papain,chABC或col'ase的化学核化.
经过治疗的IVD在动态生理负载下培养了7天.
评估包括宏观变化,糖氨基甘油 (GAG) 含量,身高下降,细胞活力和细胞外基因矩阵 (ECM) 标记物的基因表达.

主要成果:

帕帕因和原酶产生了宏观的空洞;帕帕因诱导了最显著的GAG和身高损失.
ChABC和原酶将细胞活力降低到60-70%,而帕帕因保持了接近100%的活力.
帕帕因和原酶一般降低了ECM基因表达的调节,模仿了晚期退化;chABC上调了COL2表达,反映了早期退化.

结论:

帕帕因和原酶治疗有效地模拟了先进的IVD退化,而chABC则模拟了早期退化.
牛整体IVD的化学核分解为研究一系列退行性变化的研究提供了一个多功能平台.
这些模型对于研究IVD退化潜在的治疗干预是有价值的.

关键词:

化学核聚变 (CN) 化学核聚变退化退化退化细胞外矩阵是细胞外矩阵.椎间盘之间的椎间盘.器官培养器官培养器官文化

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