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用复合乳糖杆菌 (Lactococcus lactis) 制备的基于树突细胞的疫苗通过ROS生产增强了抗原交叉呈现和抗瘤疗效

Tingting Zhang¹, Xianxian Wei¹, Yijie Li¹

¹Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, China.

Frontiers in immunology

|September 15, 2023

概括

乳球菌增强树突细胞活化和抗瘤免疫力. 这种安全的载体为改善交叉呈现和T细胞反应提供抗原,显著抑制瘤生长.

科学领域:

免疫学免疫学免疫学
微生物学微生物学
生物技术是生物技术.

背景情况:

乳球菌 (L.L) 是一种安全的细菌,具有作为传递载体的潜力.
树突细胞 (DCs) 对于启动免疫反应至关重要.
了解L.L对DCs的免疫调节作用是疫苗开发的关键.

研究的目的:

为了研究L.L对树突细胞 (DC) 激活的免疫调节作用.
探索LL影响DC成熟和抗原呈现的机制.
为了评估基于L.L的DC疫苗对瘤的疗效.

主要方法:

聚焦显微镜和流动细胞计分析了DC成熟度和抗原呈现.
调查了L.L的内部化和DCs内的共同本地化.
在B16-OVA小鼠模型中评估L.L-OVA DC疫苗的抗瘤作用.

主要成果:

通过TLR2和下游信号通路,L.L显著促进了DC成熟.
通过依赖ROS和蛋白质体的细胞体到细胞体路径,L.L增强了DC抗原的交叉呈现.
L.L-OVA DC疫苗诱导了强烈的Th1和细胞毒性T淋巴细胞反应,抑制了瘤生长.

结论:

再组合L.L作为DC疫苗的有效抗原输送系统.
基于LL的DC疫苗增强了抗原交叉呈现和抗瘤疗效.
在开发新型癌症免疫疗法方面,L.L.具有前景.

关键词:

抗瘤疗效抗瘤疗效交叉表示交叉表示基于树突细胞的疫苗有活性氧物种的反应性氧物种.再组合的乳球球菌乳酸菌.

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