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调节的折叠和结合,使用一种对刺激有反应的分子 tweezer

Sooho Ko¹, Joo-Young Kim², Jung Yeon Park³

¹Department of Materials Science and Engineering, Yonsei University Seoul 03722 Republic of Korea.

Chemical science

|September 15, 2023

概括

这项研究引入了一种新型的分子 tweezer (MT),使用β-hairpin结构来控制α-螺旋折叠和结合. 这种设计增强了的亲和力,并使生物医学应用的生物材料的刺激响应捕获和释放成为可能.

科学领域:

生物化学生物化学
分子生物学分子生物学
生物材料科学生物材料科学

背景情况:

由α-螺旋介导的蛋白相互作用在生物过程中至关重要.
控制形和结合亲和力对于治疗和诊断应用至关重要.
开发用于生物材料操纵的刺激反应系统仍然是一个重大挑战.

研究的目的:

为了开发一个基于β-hairpin (托拉链,Trpzip) 的分子 tweezer (MT).
调查MT控制α-螺旋的折叠和结合的能力.
探索MT在以刺激反应方式捕获和释放多价值生物材料方面的潜力.

主要方法:

来自p53蛋白的α-螺旋体与Trpzip在宏环结构中的结合.
使用侧链对侧链链接策略来稳定螺旋形状.
使用氧化还原刺激来诱导展开并评估螺旋性和结合亲和力的变化.
展示捕获和释放hDM2-金纳米粒子合物.

主要成果:

折叠的β-hairpin结构稳定了α-螺旋形状,增强了目标 (hDM2) 的亲和力.
红氧诱导的展开显著降低了螺旋性和结合亲和力,证明了刺激反应性.
MT有效地捕获和释放了模型多价值生物材料.
开发的MT显示了控制蛋白相互作用和检测癌症生物标志物的潜力.

结论:

基于β-hairpin的分子针提供了一种新的策略来控制α-螺旋的形和结合.
MT的刺激反应性质允许控制生物材料的捕获和释放.
这项技术有望用于蛋白质相互作用抑制和癌症生物标志物检测,包括循环瘤细胞和外体.

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