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[MiR-301a-5p调节副甲状腺激素的分泌在二次性副甲状腺功能障碍,可能通过调节感应受体]

L Liu¹, L Qian¹, P Li¹

¹Department of Breast and Thyroid Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China.

Nan fang yi ke da xue xue bao = Journal of Southern Medical University

|September 15, 2023

概括

在二次性甲状腺功能障碍症 (SHPT) 中,miR-301a-5p是上调的,可能会降低感受受体 (CaSR) 表达. 抑制miR-301a-5p会增加副甲状腺激素 (PTH) 的分泌,这表明它在SHPT的发病过程中起作用.

科学领域:

内分泌学在内分泌学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

二次性副甲状腺功能障碍症 (SHPT) 的特征是副甲状腺激素 (PTH) 分泌失调.
感应受体 (CaSR) 在调节PTH水平方面发挥着至关重要的作用.
微RNA (miRNA) 涉及到各种疾病过程,包括SHPT.

研究的目的:

为了识别下调SHPT中的CaSR的miRNAs.
为了研究这些miRNAs对PTH分泌的影响.
探索miR-301a-5p作为SHPT中的CaSR和PTH的潜在调节者.

主要方法:

正常和SHPT副甲状腺组织的全转录组测序.
生物信息预测和双露西法酶测定以确定CaSR调节小RNA.
qRT-PCR,西式涂抹和细胞培养实验用于评估miR-301a-5p,CaSR和PTH水平.

主要成果:

在SHPT组织中,miR-301a-5p被显著上调.
在SHPT中,CaSR mRNA和蛋白质表达被下调.
过度表达miR-301a-5p降低了CaSR蛋白,而抑制则增加了它;抑制miR-301a-5p显著增加了PTH分泌.

结论:

miR-301a-5p是SHPT中CaSR表达的关键调节剂.
miR-301a-5p影响PTH分泌,这表明它在SHPT病变发生过程中的作用.
向miR-301a-5p可能为SHPT提供一种新的治疗策略.

关键词:

感知的受体是感知的受体.在 miR-301a-5p 中使用.甲状腺上腺激素激素的作用二次性副甲状腺功能障碍症.

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