干扰素刺激的基因TDRD7与AMPK相互作用,并抑制其激活,以抑制病毒复制和病变发生
Sukanya Chakravarty1, Gayatri Subramanian1, Sonam Popli1
1Department of Medical Microbiology and Immunology, University of Toledo College of Medicine and Life Sciences , Toledo, Ohio, USA.
mBio
|September 15, 2023
概括
图多尔域含7 (TDRD7) 是一种新型的干扰素刺激基因,通过阻断自性来抑制病毒复制. 这一发现揭示了一个新的抗病毒途径,具有潜在的治疗应用.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 干扰素刺激基因 (ISG) 对于抗病毒防御至关重要.
- 自途径在病毒感染中起着复杂的作用.
- 图多尔域含有7 (TDRD7) 是一个新发现的ISG.
研究的目的:
- 阐明TDRD7抑制病毒复制的分子机制.
- 研究TDRD7和腺单酸盐 (AMP) 激活的蛋白激酶 (AMPK) 之间的相互作用.
- 评估TDRD7-AMPK通路的体内抗病毒作用.
主要方法:
- 蛋白与蛋白相互作用测试以确定TDRD7结合伙伴.
- 局部定向突变发生,绘制TDRD7.7的AMPK相互作用域.
- 在TDRD7缺乏的原始细胞中进行病毒复制试验.
- 在体内研究中,使用TDRD7淘汰赛小鼠挑战呼吸道病毒.
主要成果:
- TDRD7直接与AMPK相互作用,这是自的一个关键调节器.
- TDRD7的AMPK相互作用域对其抗病毒活性至关重要.
- TDRD7 缺乏导致增强AMPK激活和增加病毒复制在细胞和小鼠.
- TDRD7淘汰赛小鼠对呼吸道病毒感染的易感性增加.
结论:
- TDRD7通过抑制AMPK介导的自而起作用,作为一种新的抗病毒ISG.
- 该TDRD7-AMPK通路代表了宿主的先天抗病毒反应的关键组成部分.
- 由TDRD7衍生的可以通过向AMPK作为新型抗病毒疗法.
相关概念视频
PI3K/mTOR/AKT Signaling Pathway
3.6K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.6K
TGF - β Signaling Pathway
7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K
The JAK-STAT Signaling Pathway
9.0K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
9.0K
Immune Response Against Viral Pathogens
815
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
815
Experimental RNAi
6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
Interactions Between Signaling Pathways
6.3K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K


