在对古代人类DNA进行溶液丰富时对等位基因偏差
Roberta Davidson1, Matthew P Williams1,2, Xavier Roca-Rada1
1The Australian Centre for Ancient DNA and the Environment Institute, School of Biological Sciences, The University of Adelaide, Adelaide, South Australia, Australia.
Molecular ecology resources
|September 15, 2023
概括
古遗传学新商业套件引入了测试偏差,人工增加了遗传相似性. 这种偏见影响了种群遗传学分析,如共享漂移计算,可能会误解古代人类关系.
科学领域:
- 古遗传学是古遗传学的一部分.
- 古代DNA分析 古代DNA分析
- 人口遗传学 人口遗传学
背景情况:
- 在溶液中杂交丰富是分析古代DNA (aDNA) 的关键.
- 1240k SNP 面板已被广泛使用,但商业访问被限制到2021年.
- 新的商业套件,包括Daicel Arbor Biosciences Prime Plus套件,现在可以更广泛地获得1240k SNP缩.
研究的目的:
- 为了评估Daicel Arbor Biosciences的Prime Plus套件,用于在古代样本中1240k SNP丰富.
- 识别和描述Prime Plus套件引入的任何系统偏见.
- 评估已识别的偏见对人口遗传学和遗传关系分析的影响.
主要方法:
- 使用戴塞尔·阿伯生物科学Prime Plus套件丰富了来自三大洲的132个古老DNA样本.
- 系统偏差检测和量化.
- 对偏差的比较分析与传统的1240k丰富试验.
- 对人口遗传学指标 (例如,主要组件分析,f统计) 和遗传相关性 (READ) 的偏差影响评估.
主要成果:
- 在Prime Plus套件中发现了系统性测试偏差,增加了样品之间的遗传相似性.
- 这种偏差独立于批量效应,导致f-统计中的人工过量共享漂移.
- 大陆内部的人口关系被Prime Plus数据不准确地表示.
- 在READ分析中,偏差更微妙,但仍可能导致误导性解释.
结论:
- 戴塞尔·阿伯生物科学Prime Plus套件引入了一个影响古遗传学分析的重大偏差.
- 人工共享漂移掩盖了真正的人口结构,特别是在大陆内.
- 研究人员必须在广泛采用之前严格验证新的1240k丰富技术,以确保数据完整性.
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