基于多西洛的分子逻辑门,用于双通道可视化在cuproptosis期间线粒体pH和硫酸盐变化的分子逻辑门
Fanfan Wang1, Ying Lan1, Yujing Zuo1
1School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Jinan, Shandong 250022, P.R. China.
Analytical chemistry
|September 15, 2023
概括
研究人员开发了一种新的光探针,用于监测 mitochondria 中的pH和二氧化硫 (SO2) 水平在细胞死亡途径 cuproptosis 期间. 该工具有助于理解细胞应激反应,并开发新的治疗策略.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 分子探头分子探头
背景情况:
- 质亡是一种细胞内铜诱导的调节细胞死亡途径,涉及线粒体功能障碍.
- 线粒体在细胞损伤和免疫反应期间的代谢调节中至关重要.
- 线粒体内pH值和二氧化硫 (SO2) 水平是cuproptosis调节的关键指标.
研究的目的:
- 开发一种光探针,能够同时检测在cuproptosis期间的线粒体内pH和SO2的变化.
- 建立一个分子逻辑门探测器,用于实时监测cuproptosis中的细胞状态.
- 研究 mitochondrial 内硫酸盐和质子在cuproptosis过程中的作用.
主要方法:
- 利用一个功能性的聚氧平台来创建一个分子逻辑门探测器 (P-pH-SO2).
- 采用双通道检测模式来监测pH和SO2水平.
- 根据探针的逻辑关系定义了一个新的功能,以反映在elasclomol诱导的cuproptosis期间的细胞状态.
主要成果:
- 该P-pH-SO2探头表现出线粒体硫酸盐的快速激活,诱导绿色光.
- 线粒体中的质子存在导致红色光的灭.
- 探测器成功地以逻辑关闭的方式监测了线粒体内pH和SO2的变化.
结论:
- 开发了一种新的逻辑门分子探测器,用于同时检测线粒体pH和SO2.
- 这种探测器提供了一种多功能策略,用于监测硫酸盐和H+在cuproptosis中的作用.
- 该研究扩大了分子逻辑门和光聚氧在生物研究中的应用.
相关概念视频
01:19Ion Channels
The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow specific...
Ion channels are specialized integral membrane proteins on the plasma membrane that allow specific...
01:24Non-gated Ion Channels
Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
01:19Ligand-gated Ion Channels
Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that include the...
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that include the...
01:12Mechanically-gated Ion Channels
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
01:24Non-gated Ion Channels
Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
01:12Mechanically-gated Ion Channels
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...