相关实验视频

Updated: Jul 28, 2026

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment
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Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Published on: June 2, 2020

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离子交换色谱作为一种简单且可扩展的方法,可以从条件介质中分离出生物活性的小细胞外囊泡

Ricardo Malvicini1,2,3,4, Diego Santa-Cruz1, Anna Maria Tolomeo3,4,5

  • 1Laboratorio de Regulación Génica y Células Madre, Instituto de Medicina Traslacional, Trasplante y Bioingeniería (IMeTTyB), Universidad Favaloro-CONICET, Buenos Aires, Argentina.

PloS one
|September 15, 2023
PubMed
概括

研究人员开发了一种可扩展的染色学方法,以从细胞培养基中分离细胞外囊泡 (EV). 这种方法产生了纯粹的,生物活跃的EV,适合临床应用和治疗用途.

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Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Ion-Exchange Chromatography

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Affinity Chromatography

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