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过度FSH剂量的卵巢刺激会导致小卵巢储备小的累积细胞和卵细胞功能障碍

Kaitlin R Karl¹, Peter Z Schall¹, Zaramasina L Clark¹

¹Department of Animal Science, Reproductive and Developmental Sciences Program, Michigan State University, East Lansing, MI, USA.

Molecular human reproduction

|September 15, 2023

概括

过度的卵泡刺激激素 (FSH) 剂量会破坏牛卵细胞的成熟和细胞功能. 这导致过早扩张和卵细胞质量差,影响体外受精的成功.

科学领域:

生殖生物学生殖生物学
动物科学动物科学
内分泌学在内分泌学.

背景情况:

在小卵巢储备母牛 (SORH) 卵巢刺激期间高剂量的卵泡刺激激素 (FSH) 诱导卵泡过度刺激失调 (FHD).
假设FSH失调会损害累积细胞功能和卵细胞成熟,影响信号通路.

研究的目的:

研究过度FSH对累积细胞基因表达和卵细胞成熟的影响.
为了确定过多的FSH是否会改变hCG刺激前后或体外成熟 (IVM) 期间的累积细胞形态和卵细胞成熟.

主要方法:

对过度FSH治疗的小母的累积细胞中差异表达基因 (DEGs) 的分析.
在各种刺激条件下 (hCG前,hCG后,IVM) 评估累积细胞形态和卵细胞核成熟.

主要成果:

过度的FSH增加了参与性腺激素反应,生存,扩张和卵细胞成熟的17个累积DEGs的表达.
过度的FSH诱导过早的累积膨胀和卵细胞成熟,而hCG后成熟被抑制.
有过早扩张的积分细胞的卵细胞显示出体外受精 (IVF) 和IVM的能力降低.

结论:

过度的FSH会扰乱积分细胞和卵细胞的功能,导致积分细胞过早扩张和卵细胞成熟失调.
这导致质量差的积卵细胞复合体,可能被误认为适合辅助生殖技术 (ART).
卵巢刺激方案应仔细管理FSH剂量,以优化对ART的卵细胞和累积细胞功能.

关键词:

在累积细胞中的FSH标基因.累积oocyte 复合体中的一个.累积功能的调节失调.过度增加的FSH.卵细胞检索卵细胞检索过早的累积扩张过早的累积扩张.介质变化重启的过程.小卵巢储备是一个小卵巢储备.超级排卵是一种超级排卵.

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