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干旱原始化调节ABF,GRF,相关的微RNA,并在小中在热应激期间诱导代谢调整

Sumandeep Juneja¹, Rashmi Saini¹, Abdul Mukit²

¹Centre for Biosciences, School of Basic and Applied Sciences, Central University of Punjab, Bathinda, 151401, India.

Plant physiology and biochemistry : PPB

|September 15, 2023

概括

这项研究确定了豆中对酸反应的基因 (ABF,GRF) 和微RNA. 干旱原料和热应激揭示了这些因素如何调节植物反应,耐受品种表现出更好的适应性.

科学领域:

植物科学植物科学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

干旱和热应激会导致植物细胞功能障碍.
酸 (ABA) 是一种关键的压力调节剂,其响应基因由ABRE结合因子 (ABF) 和G盒调节因子 (GRF) 控制.

研究的目的:

在desi和kabuli小豆中识别ABF,GRF和准miRNA.
为了验证它们在对干旱的反应中所起的作用,其次是高温压力.
为了研究ABF-GRF蛋白-蛋白相互作用.

主要方法:

在小豆中对ABF和GRF进行全基因组分析.
在对比的小品种 (PBG1,PBG5) 上进行干旱原料和热应激处理 (32°C,35°C,38°C).
物理生物化学分析,基因表达特征 (ABF,GRF,miRNA),GC-MS代谢物分析,以及蛋白质-蛋白质对接研究.

主要成果:

在desi和kabuli小豆中分别鉴定了9个和11个ABF和11个GRF,它们具有保存的基因结构.
确定了米R172和miR396的目标分别是ABF和GRF.
干旱原料降低了miR172和miR396的调节,提高了ABF和GRF的调节;ABF3-GRF14相互作用需要ABF3酸化. 代谢物分析显示了87种累积的代谢物.
耐受性品种PBG5对压力表现出更好的反应.

结论:

ABF,GRF和miRNA在豆对干旱和热应激的反应中起着至关重要的作用.
干旱原始化通过调节这些关键调节基因的表达来增强耐受性.
压力的严重程度和持续时间显著影响细胞反应,观察到种类特定的耐受性变化.

关键词:

在ABF ABF ABF 豆是什么意思豆是什么意思格力福格力福格力福格力福格力福格格格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格力福格热应力是一种热应力.代谢物的代谢物.启动启动启动这是一个小RNARNA.

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