直接招募 Mis18 进入相间螺旋杆体,促进了 CENP-A 染色体组合
Nitobe London1, Bethan Medina-Pritchard1, Christos Spanos1
1Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, Edinburgh EH9 3BF, Scotland, UK.
Current biology : CB
|September 15, 2023
概括
螺旋杆体 (SPBs) 将Mis18复合物招募到裂变酵母中的中位素中,独立于M18BP1. 这种由SPB介导的招募对于确定CENP-A染色体和中间体的身份至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 染色体生物学 染色体生物学
背景情况:
- 哺乳动物中的中间体标识依赖于CENP-A染色体,由Mis18复合体 (Mis18C) 招募的HJURP补充.
- 在缺乏M18BP1的物种中,例如裂变酵母中,Mis18C招募机制尚不清楚.
- 在CENP-A的补充过程中,裂变酵母的中间体聚集在螺旋杆体 (SPB) 上,Mis18C也在其中定位.
研究的目的:
- 调查SPBs在Mis18C招募中对裂变酵母中CENP-A染色体组合的作用.
- 为了阐明在缺乏M18BP1的物种中Mis18C招募的机制.
- 为了了解核组织如何影响中心体的身份.
主要方法:
- 使用裂变酵母遗传学和显微镜研究了SPBs和Mis18C之间的相互作用.
- 分析了SPB主要组成部分Sad1在Mis18C招募中的作用.
- 研究了SPB介导的Mis18C招募对CENP-ACnp1染色体建立和维护的功能后果.
主要成果:
- SPBs通过直接结合到Sad1.1,通过招募Mis18通过直接结合到Sad1.1,将Mis18C集中在离心体附近.
- 在建立CENP-ACnp1染色蛋白时,Sad1介导的Mis18C招募是必不可少的.
- 这一途径与CENP-C介导的招聘并行运行,并且独立于Sad1驱动的中位素聚类.
结论:
- 在G2期间,SPBs作为Mis18C招募和中间体聚类的非染色体支架.
- 通过SPB介导的Mis18的招募管理了新的CENP-ACnp1染色体组合.
- 通过SPB-Mis18相互作用,核空间组织会影响中心体的身份.
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