维蒂斯Myb14通过激活脂质转移蛋白基因表达和活性氧物种清理,赋予寒冷和干旱耐受性
Linchuan Fang1, Zeming Wang2, Lingye Su3
1Wuhan Academy of Agricultural Science, Wuhan, China.
Gene
|September 15, 2023
概括
维蒂斯Myb14通过调节应激反应基因和增强抗氧化活性来增强植物对寒冷和干旱的耐受性. 这种转录因子对于葡萄酒适应环境挑战至关重要.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
- 遗传学 是一个遗传学.
背景情况:
- R2R3 Myb转录因子调节各种植物过程,包括耐压力.
- 维蒂斯Myb14最初与葡萄藤中的白醇合成有关.
- 了解葡萄酒对非生物压力的反应对农业至关重要.
研究的目的:
- 调查Vitis Myb14在葡萄树中的非生物应激耐受性中的作用.
- 为了比较耐寒品种 (Vitis amurensis) 和耐寒品种 (V. vinifera) 的应激反应.
- 阐明VaMyb14介导的压力耐受性背后的分子机制.
主要方法:
- 在非生物和激素治疗下进行基因表达分析.
- 在Arabidopsis thaliana中,VaMyb14的过度表达.
- 测量抗氧化酶活性和MDA含量.
- 转基因植物和葡萄藤的微阵列和mRNA测序分析.
主要成果:
- VaMyb14表达对寒冷,干旱和盐分压力有反应,在V. amurensis中反应更为明显.
- 在Arabidopsis中过度表达VaMyb14,增强了寒冷和干旱耐受性,增加了抗氧化剂活性 (特别是POD),并降低了MDA水平.
- 微阵列和测序数据显示,VaMyb14对ABA代谢,信号转导基因 (LTPs,PP2Cs,RD29B,COR78) 和ROS清理途径进行上调调节.
结论:
- 维蒂斯Myb14是葡萄藤非生物应激反应的关键调节者.
- 通过调节ABA信号和抗氧化剂防御,VaMyb14增强了对寒冷和干旱压力的耐受性.
- 这些发现表明Vitis Myb14在改善作物弹性和植物素生产方面的潜在作用.
相关概念视频
Responses to Heat and Cold Stress
13.6K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.6K
Regulation of Transpiration by Stomata
28.4K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
28.4K
Introduction to Plant Diversity
44.9K
From Water to Land
44.9K
Adaptations that Reduce Water Loss
25.7K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
25.7K
Gene Regulation During Sporulation
34
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
34
Transgenic Plants
7.3K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.3K


