在RAW264.7细胞中,U(VI) 暴露会诱导亡和亡
Zhuna Yan1, Yan Zhang1, Liang Du2
1School of Medicine, Anhui University of Science and Technology, Huainan 232001, PR China; Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, PR China.
Chemosphere
|September 15, 2023
概括
(VI) 暴露会损害细胞,导致氧化应激,亡和热亡. 这项研究详细介绍了RAW264.7细胞中的毒性机制,揭示了其免疫毒性作用.
科学领域:
- 环境科学 环境科学
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- (VI) 污染对环境和健康构成重大风险.
- 了解毒性机制对于风险评估和缓解至关重要.
研究的目的:
- 在RAW264.7细胞上研究的免疫毒性.
- 阐明 (VI) 诱导的细胞死亡所涉及的细胞和分子途径.
主要方法:
- RAW264.7细胞在24小时和48小时内暴露在不同度的中.
- 评估了细胞活力,反应性氧物种 (ROS) 生产,线粒体膜潜力 (MMP) 和超氧化物脱酶 (SOD) 活性.
- 利用流式细胞计 (Annexin-V/PI染色) 和西式涂抹来分析亡,亡标记物和MAPK通路激活.
主要成果:
- 暴露于 () 降低了细胞活力,增加了细胞内ROS,破坏了MMP,并提高了SOD活性.
- ((VI) 诱导了晚期的亡和烧亡,通过增加的Annexin-V/PI染色,LDH释放和GSDMD表达来证明.
- 观察到Bcl-2的下调和Bax表达的上调,以及MAPK信号通路 (Ras,p38,JNK,ERK1/2) 的激活.
结论:
- (VI) 通过亡和热亡在RAW264.7细胞中诱导显著的免疫毒性.
- 该MAPK通路在引发的细胞死亡中起着关键作用.
- 这项研究为进一步研究毒性提供了理论框架.
相关概念视频
Mutations
38.2K
Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
38.2K
The Extrinsic Apoptotic Pathway
6.4K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.4K
The Intrinsic Apoptotic Pathway
6.6K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.6K
Nucleotide Excision Repair
3.5K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
3.5K
Apoptosis
11.6K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
11.6K


