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定量聚合酶连锁反应用于估计贝类生产水域中毒性微藻的丰度

Jonathan S McQuillan¹, Ahmed Alrefaey¹, Andrew D Turner²

¹Ocean Technology and Engineering, National Oceanography Centre, European Way, Southampton, SO14 3ZH, United Kingdom.

|September 15, 2023

概括

定量聚合酶连锁反应 (qPCR) 提供了一种更快的方法来检测贝类中的有害藻类繁殖 (HAB) 和相关毒素. 这项研究表明,qPCR精确地追踪产生毒素的藻类,如Dinophysis accuminata,这可能有助于改善海鲜安全监测.

科学领域:

海洋生物学海洋生物学
生态毒理学生态毒理学
分子生物学分子生物学

背景情况:

海洋微藻可以通过食用海鲜,特别是双贝类,产生对人类健康有害的生物毒素.
目前对有害藻类繁殖 (HAB) 和毒素的监测方法耗时,对当局来说延迟了结果.
定量聚合酶链反应 (qPCR) 为检测浮游生物DNA提供了一个快速的替代方案,但不是标准实践.

研究的目的:

评估qPCR在监测贝类中的有害藻类及其相关毒素方面的有效性.
为了比较qPCR与传统显微镜用于植物浮游生物监测.
根据官方控制条例,评估qPCR在常规贝类监测中的潜力.

主要方法:

在六个月的时间里,每周从圣奥斯特尔湾 (St Austell Bay) 取样,这是一个已知的HAB热点.
显微镜和qPCR用于测量水中的藻类细胞丰度.
在肉中量化LC-MS/MS的脂友毒素 (酸组).
qPCR测量了亚历山大种类的DNA. 和Pseudo-nitzschia spp. 这种植物.

主要成果:

藻类细胞丰富度增加与贝中较高的甲酸组毒素度相关.
qPCR检测出与毒素事件相对应的Dinophysis accuminata DNA的增加.
qPCR在春天发现了D. accuminata的DNA,当显微镜没有时,也检测到了亚历山大 spp. 和Pseudo-nitzschia spp. 这种植物. 鲜花. 鲜花. 这些鲜花.

结论:

qPCR是快速检测有害藻类的有希望的工具,可以补充或潜在地取代传统方法.
这项研究强调了qPCR能够检测微镜无法检测的藻类增加的能力,从而更快地了解潜在的风险.
qPCR可以显著提高贝类安全监测计划的及时性和有效性.

关键词:

藻类的开花藻类的开花恐龙溶解 (Dinophysis) 是一种早期预警早期预警官方控制官方控制 qPCRR 是一个很好的方法.

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