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可转移的元素驱动了元动物指基因的进化
Jonathan N Wells1, Ni-Chen Chang2, John McCormick2
1Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14850, USA; jnw72@cornell.edu cf458@cornell.edu.
Genome research
|September 15, 2023
概括
与可移植元素 (TE) 的基因组冲突推动了动物间指基因 (ZNF) 的演化. 此前在哺乳动物中观察到的这种关系现在在鱼类中得到证实,突出了ZNFs.
科学领域:
- 进化基因组学是进化的基因组学.
- 分子进化是分子进化的过程.
- 动物的植物学.
背景情况:
- Cys2-His2指基因 (ZNFs) 是甲基动物中最大的转录因子家族,以动态进化变化而闻名.
- 对于哺乳动物的克鲁佩尔相关盒子ZNFs (KZNFs),可移植元素 (TEs) 在ZNF进化中的作用已经确立,但其更广泛的意义尚不清楚.
研究的目的:
- 调查基因组与TEs的冲突是否是跨元动物中ZNF多样化的一般驱动因素.
- 探索鱼类N端指关联 (FiNZ) 域ZNF子家族的功能和演变.
主要方法:
- 分析了3221个甲动物基因组组合,以关联反元素和ZNF副本数.
- 在各种动物物种中计算预测ZNF-TE结合偏好.
- 斑马鱼的功能研究,包括morpholino knockdown,以评估FiNZ-ZNF在TE抑制中的作用.
主要成果:
- 在7500万年的元动物进化中,反元素和ZNF复制数之间存在显著的相关性.
- 跨多种物种的ZNFs表现出对TEs的优先结合.
- 斑马鱼FiNZ-ZNFs在发育早期得到表达,它们的抑制导致TE脱压,类似于KZNFs.
结论:
- 与TEs的基因组冲突是动物中ZNF多样化背后的主要驱动力.
- ZNFs和TEs之间的共同进化关系是整个元动物进化过程中保存的特征,不仅限于KZNFs.
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