结构和特征Tremella fuciformis多糖/乳清蛋白分离纳米颗粒的结构和特征,用于持续释放黄素
Jiaqi Shang1, Yang Liu2, Jiaxin Kang3
1Key Laboratory of Bionic Engineering, College of Biological and Agricultural Engineering, Jilin University, Changchun, China.
Journal of the science of food and agriculture
|September 15, 2023
概括
特雷梅拉形糖多糖 (TFPs) 增强了乳清蛋白分离物 (WPI) 纳米颗粒的稳定性,用于黄素 (CUR) 输送. 这些TFP-WPI-CUR纳米粒子显示出高封装效率和持续释放,扩大了CUR在食品和药品中的应用.
科学领域:
- 食品科学 食品科学 食品科学
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- 乳清蛋白分离物 (WPI) 纳米粒子提供黄素 (CUR) 传递的潜力,但缺乏稳定性.
- 特雷梅拉形菌多糖 (TFPs) 在提高WPI稳定性方面表现有前途.
研究的目的:
- 系统地描述和分析具有不同CUR含量的TFP/WPI纳米颗粒的结构.
- 评估CUR装载TFP-WPI纳米粒子的稳定性和释放性质.
主要方法:
- 用CUR.装载的TFP/WPI纳米颗粒的制备和表征.
- 评估封装效率,粒子大小,泽塔潜力以及在各种条件下的稳定性.
- 使用观察WPI结构变化的技术进行结构分析.
- 在体外释放研究以确定CUR释放动力学.
主要成果:
- 实现了最高的CUR封装效率98.8%和47.88%的负载含量.
- 优化的TFP-WPI-CUR纳米颗粒的粒子大小为653.67nm,泽塔电位为-38.97mV.
- 纳米粒子在各种盐离子和pH条件下21天表现出极好的稳定性.
- 结构分析显示TFP和CUR诱导的无形WPI结构具有降低的β-sheet含量.
- 在体外释放研究证实了从TFP-WPI-CUR纳米颗粒中持续释放CUR的行为.
结论:
- TFP-WPI纳米粒子作为CUR传递的有效载体.
- 开发的纳米粒子增强了CUR的稳定性,并使持续释放成为可能.
- 这些发现表明TFP-WPI-CUR纳米颗粒在功能性食品,食补充剂和药品中的广泛应用.
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