人类骨肌肉组织芯片的自主有效载荷揭示了由于太空飞行的纤维类型和代谢基因表达的变化
Maddalena Parafati1, Shelby Giza1, Tushar S Shenoy1
1Department of Pharmacodynamics, College of Pharmacy, University of Florida, Gainesville, FL, 32610, USA.
NPJ microgravity
|September 15, 2023
概括
太空飞行加速了肌肉衰老,模仿了肉症. 飞往国际空间站的三维肌肉组织芯片揭示了肌肉细胞的分子变化,为衰老和太空诱导的肌肉缩提供了洞察力.
科学领域:
- 太空生物学空间生物学
- 细胞生物学 细胞生物学
- 生物技术是生物技术.
背景情况:
- 太空飞行诱导肌肉缩,与与年龄相关的肉症有相似之处.
- 微生理系统能够在极端环境下对人体组织变化的建模.
- 研究空间诱导的肌肉变化的分子机制可以为sarcopenia研究提供信息.
研究的目的:
- 通过使用3Dmyobundles来研究暴露在太空飞行中的肌肉细胞中的分子变化.
- 为了比较太空飞行对年轻人和老年人的肌肉组织的影响.
- 评估 CubeLabTM 自主有效载荷在空间生物学研究中的实用性.
主要方法:
- 利用来自年轻和老年人肌肉活检的3D肌肉组.
- 在国际空间站 (ISS) 飞行中,集成的myobundles 集成到一个自主 CubeLabTM 有效载荷中.
- 进行了全球转录基因RNA-Seq分析,比较太空飞行和地面控制样本.
主要成果:
- 在太空飞行样本中观察到与肌细胞增殖和肌肉分化相关的转录的下调.
- 在暴露于空间的老年成年肌肉团中,肌肉代谢途径的独特下调.
- 在太空中飞行的年轻成年肌团中观察到的炎症途径基因的下调.
结论:
- 肌肉组织芯片平台有效地模拟了太空飞行对肌肉生物学的细胞自主影响.
- 这些发现提供了关于太空飞行诱导的缩和缩症之间的分子平行线的见解.
- 自主CubeLabTM有效载荷为微重力组织芯片实验提供了一种可行的方法.
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