一个线性和循环的双形态非编码RNA参与了Bacillus altitudinis中的氧化应激耐受性
Ting-Ting He1,2, Yun-Fan Xu1, Xiang Li1
1Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
Nature communications
|September 15, 2023
概括
循环RNAs (circRNAs) 在细菌中发现,其中一种形式 (DucS) 帮助Bacillus altitudinis在过氧化压力下. 细菌circRNA的存在因物种而异,它们的功能在很大程度上仍未知.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
背景情况:
- 循环RNA (circRNAs) 在真核生物中得到了充分的记录,但它们在细菌中的存在和功能在很大程度上尚未被探索.
- 了解细菌circRNA可以揭示新的调节机制和应激反应途径.
研究的目的:
- 研究细菌中循环RNA的存在和功能,特别关注Bacillus altitudinis.
- 在其他细菌物种中识别潜在的circRNA候选者,并评估circRNA形成的保存.
主要方法:
- RNA测序和生物信息学分析以确定30种细菌物种的circRNA候选者.
- 在Bacillus altitudinis,Bacillus paralicheniformis,Bacillus subtilis和Escherichia coli中对选定的circRNA候选物的实验验证.
- 分析线性DucSRNA在细菌应激反应中的作用及其与循环DucS形成的关系.
主要成果:
- 在Bacillus altitudinis中发现了一种双形态调节RNA (DucS),存在于线性和圆形形式,存在于Bacillus altitudinis.
- 线性DucS通过上调htrA基因来提高对过氧化压力的耐受性.
- 循环DucS形成可以降低线性DucS的水平,并且在多种细菌物种中发现了circRNA候选物种,尽管它们并不普遍保存.
结论:
- 循环RNA存在于各种细菌物种中,挑战了关于它们真核细胞特异性的先前假设.
- 在Bacillus altitudinis中发现的DucSRNA显示了压力反应的调节作用,其线性和圆形形式具有不同的功能.
- 细菌中circRNA形成的机制及其特定的生物功能在各个物种中并未得到保存,需要进一步研究.
相关概念视频
Types of RNA
63.9K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.9K
Translational Regulation
42
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
42
Transcriptional Regulation: Riboswitches
61
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
61
Stringent Response in E. coli
31
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
31
Other Stress Responses in Bacteria
33
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
33
Riboswitches
8.2K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.2K


