管功能和形态在没有标记的脏中使用三维动态光学连贯断层扫描揭示了脏的功能和形态
Pradipta Mukherjee1, Shinichi Fukuda2,3, Donny Lukmanto4
1Computational Optics Group, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Scientific reports
|September 15, 2023
概括
动态光学连贯断层扫描 (DOCT) 在脏阻塞的小鼠模型中可视化管功能. 这种无标签的成像方法揭示了管结构和动态变化,表明病.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學專業.
- 生物医学成像技术 生物医学成像技术
- 光学工程是指光学工程.
背景情况:
- 管具有独特的代谢和功能特征.
- 脏疾病可以显著改变这些生理特征.
- 无标签成像技术对于非侵入性地研究功能至关重要.
研究的目的:
- 开发和应用无标签,三维 (3D) 动态光学连贯断层扫描 (DOCT) 用于管的功能成像.
- 为了研究管结构和功能在脏阻塞的小鼠模型的变化.
- 评估DOCT在表征病状态中的实用性.
主要方法:
- 正常和受阻的小鼠脏的ex vivo 3D DOCT成像 (7-14天尿管结合).
- 采用了两个DOCT方法:用于缓慢活动的对数强度变量 (LIV) 和用于快速活动的复杂相关性.
- 采用1.3微米扫描源OCT系统与光扫描获得的数据.
主要成果:
- 在DOCT投影图像中,正常的管看起来是截然不同的,复杂的管状结构.
- 阻塞的脏缺乏可观察到的解剖管状结构.
- 阻塞的脏在围皮层区域表现出表面的高动态外观.
结论:
- 3D DOCT提供了管的无标签功能成像.
- 尿道阻塞导致正常管道形态的丧失和动态的改变.
- 使用DOCT的体积度LIV是研究疾病期间功能的一个有前途的工具.
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