通过ESI-HRMS和定制的质量缺陷软件工具,通过优化样品准备技术和分析,从各种来源和处理过程中表征素
Dane R Letourneau1, Bryan P Marzullo2, Anastasia Alexandridou1
1Department of Chemistry, Humboldt University Berlin, 12489, Berlin, Germany.
Analytical and bioanalytical chemistry
|September 15, 2023
概括
本研究详细介绍了优化样品制备协议,用于分析复杂的素混合物,使用电喷离子高分辨率质谱法 (ESI-HRMS). 这些方法提高了数据质量,并使不同类型的素样本能够更好地表征.
科学领域:
- 分析化学 分析化学
- 生物质科学 生物质科学
- 频谱学是一种光谱学.
背景情况:
- 精确分析复杂的天然混合物,如素,需要仔细的样本准备.
- 现代质谱技术,包括猎枪MS,往往需要预处理以获得最佳性能.
- 降解的红素样本由于其不同来源和加工而存在独特的挑战.
研究的目的:
- 为各种降解的素样品建立强大的样本准备协议.
- 为了优化电子喷射电离 - 高分辨率质谱 (ESI-HRMS) 条件用于素分析.
- 为复杂的素数据集开发有效的数据可视化方法.
主要方法:
- 开发和应用各种红素来源的样品制备技术.
- 使用电喷气电离 - 高分辨率质谱仪 (ESI-HRMS) 在负和正模式下进行分析.
- 数据转换成质量缺陷空间使用R脚本和星座软件进行可视化.
主要成果:
- 建立了针对四种不同的红素类型的优化样品制备协议.
- 证明了质量缺陷分析对可视化样本变化的实用性.
- 创建了信息丰富的HRMS数据集,用于全面的素表征.
结论:
- 优化的ESI-HRMS协议为分析复杂的降解基素提供了基础.
- 质量缺陷可视化有助于理解样品制备和电离效应.
- 开发的方法有助于更深入地表征多样化的木质素结构.
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