在地下水系统中固和缩生物多样性
Xiaohan Liu1,2, Ping Li3,4, Helin Wang1,2
1State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, PR China.
The ISME journal
|September 15, 2023
概括
生物固化是补充地球的关键过程. 这项研究表明,它是地下水中的重要,但尚未研究的来源,由多样化的微生物群落驱动.
科学领域:
- 环境微生物学 环境微生物学
- 地质化学 地质化学
- 生物地质化学循环的过程
背景情况:
- 生物固定 (BNF) 对于全球循环至关重要,补充生物圈的损失.
- 在地下水系统中BNF的理解很少,这限制了我们对地下动态的了解.
研究的目的:
- 为了调查Hetao平原地下水中的Diazotrophs的活动,丰富性和社区组成.
- 评估BNF在这种特定的地下水环境中对循环的贡献.
主要方法:
- 使用了15N2追踪,逆转录定量PCR (RT-qPCR) 和元基因组/元转录组分析.
- 分析了在现场地下水,缩物和隔离物中的二氧化的活性,并进行了单细胞验证.
- 与溶解氧,Fe(II) 和NH4+等环境参数相关联的二氧基因表达.
主要成果:
- 证实BNF是Hetao平原地下水中不可忽视的源,其活性在9.5-585.4nmol N L-1h-1.1之间.
- 糖尿病的丰度 (nifH基因表达) 有显著的变化 (3.4 × 103到1.2 × 106副本L−1) 并与环境因素相关.
- 地下水二氧化主要是有氧生物/选择性无氧生物,包括像Stutzerimonas和Pseudomonas这样的属,活跃的成员倾向于减少条件和不同的代谢作用.
结论:
- 在赫塔奥平原地下水的地化学循环中,有着重要的作用.
- 了解食动物群落及其活动对于理解地下生物地质化学过程至关重要.
- 对地下水BNF进行进一步的研究是有必要的,以充分了解其对的可用性和生态系统功能的影响.
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