Circ-PRMT5通过miR-7-5p/KLF4轴刺激威尔姆斯瘤的增殖能力
Jing Zhang1, Yingyu Quan2, Xiaoxia Su3
1Department of General Surgery, Hainan Women and Children's Medical Center, Haikou, China. Zhangjing_623@126.com.
Cellular and molecular biology (Noisy-le-Grand, France)
|September 16, 2023
概括
像circ-PRMT5这样的循环RNA (CircRNAs) 在威尔姆斯瘤中高度表达,促进癌症的发展. 这项研究揭示了circ-PRMT5激活miR-7-5p/KLF4轴,驱动瘤细胞的增殖和进展.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 循环RNAs (CircRNAs) 越来越多地被认为是它们在哺乳动物生物学和疾病中的作用,特别是在调节瘤细胞行为方面.
- 威尔姆斯瘤是一种常见的儿科癌,具有复杂的调节机制,尚未完全理解.
- 了解CircRNAs在威尔姆斯瘤发病过程中的特定作用,对于开发有针对性的治疗策略至关重要.
研究的目的:
- 为了研究circ-PRMT5在威尔姆斯瘤组织中的表达模式.
- 确定circ-PRMT5对威尔姆斯瘤细胞增殖和发育的功能影响.
- 为了阐明涉及circ-PRMT5/miR-7-5p/KLF4轴在威尔姆斯瘤进展中的分子机制.
主要方法:
- 在威尔姆斯瘤样本中定量检测circ-PRMT5表达.
- 在体外测试包括细胞计数套件-8 (CCK-8),殖民地形成和5-乙烯基-2'-脱氧氨 (EdU) 以评估细胞增殖.
- 路西法酶记者测试验证了circ-PRMT5/miR-7-5p/KLF4信号通路内的相互作用.
- 救援实验以证实已识别的轴在瘤发育中的作用.
主要成果:
- 发现Circ-PRMT5在威尔姆斯瘤样本中与正常脏组织相比显著上调.
- 在威尔姆斯瘤患者中,circ-PRMT5的更高表达水平与晚期瘤分期相关.
- 敲除circ-PRMT5显著抑制了威尔姆斯瘤细胞的增殖和殖民地形成能力.
- 路西法酶测定证实了circ-PRMT5,miR-7-5p和KLF4之间的直接相互作用,确定了circ-PRMT5/miR-7-5p/KLF4轴.
- 救援实验表明,circ-PRMT5通过激活这个轴来促进威尔姆斯瘤的发展.
结论:
- 在威尔姆斯瘤中,circ-PRMT5是致癌的,表现出与瘤阶段相关的高表达.
- Circ-PRMT5促进了威尔姆斯瘤细胞的增殖和恶性进展.
- 环-PRMT5的致癌功能通过miR-7-5p/KLF4信号通路进行介导,突出显示了潜在的治疗标.
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