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诺比林调节了下甲状腺状细胞癌的侵袭,迁移和转移

Marisol Rosas-Martínez¹, José Antonio González Rosales², Gloria Gutiérrez-Venegas³

¹Biochemistry Laboratory of the Division of Graduate Studies and Research, Faculty of Dentistry, National Autonomous University of Mexico, Mexico City 04510, Mexico. marisol.rosas.m@outlook.com.

Cellular and molecular biology (Noisy-le-Grand, France)

|September 16, 2023

概括

诺比素 (NOB) 通过减少细胞增殖,迁移和侵入性,有效地抑制下甲状腺皮细胞癌的进展. 这种天然化合物向关键信号通路,为这种攻击性癌症提供潜在的治疗益处.

科学领域:

在瘤学瘤学.
药理学药理学是指药理学的学科.
细胞生物学细胞生物学

背景情况:

头部和部状细胞癌,特别是下,在诊断时呈现出晚期疾病和营养不良的不良预后.
众所周知,像诺比丁 (5,6,7,8,3',4'-Hexamethoxyflavone) (NOB) 这样的聚甲类黄可以抑制癌细胞的增殖和迁移.
在下甲状腺状细胞癌中NOB的治疗潜力仍然未被探索.

研究的目的:

调查诺比丁 (NOB) 对Fadu细胞系的增殖,迁移和侵入性的影响,这是下支柱状皮细胞癌的模型.
阐明NOB的作用背后的分子机制,重点关注蛋白激酶信号通路.

主要方法:

用不同度的NOB处理了FaDu细胞.
细胞增殖被评估使用标准测试.
细胞迁移使用Transwell测试进行了评估.
蛋白激酶酸化 (ERK,Akt,PI3K) 通过西部斑分析确定.
用ELISA测量了血管内皮生长因子 (VEGF) 水平,这表明了转移潜力.

主要成果:

在15微米和40微米的度下,诺比丁 (NOB) 显著抑制了FADU细胞增殖的40%.
50μM的NOB度降低了细胞迁移和诱导的亡.
NOB治疗导致了酸化细胞外信号调节激酶 (p-ERK),蛋白激酶B (p-Akt) 和氨基酸 3-激酶 (p-PI3K) 的下调.

结论:

诺比丁 (NOB) 显示出对下状状细胞癌细胞 (FaDu) 显著的抗癌作用.
通过NOB观察到的增殖,迁移和诱导亡的抑制与关键蛋白激酶 (包括ERK,Akt和PI3K) 的下调有关.
这些发现表明,NOB是下甲状腺状细胞癌的潜在治疗剂,需要进一步调查.

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