维伦斯分泌的效应剂Uv1809通过增强OSSRT2介导的基因组脱乙烯化来抑制大米免疫力
Xiaoyang Chen1, Chen Liu1, Hailin Wang1
1Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Agricultural University, Hefei, China.
Plant biotechnology journal
|September 16, 2023
概括
一种新发现的米蛋白Uv1809,通过抑制植物免疫力来促进疾病. 准这种蛋白质或增强组织素脱乙酶OsSRT2为米疾病耐药性提供了新的策略.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 由Ustilaginoidea virens引起的米虚假污垢是对大米 (Oryza sativa) 生产的重大全球威胁.
- 乌斯蒂拉基诺idea virens-rice相互作用和病原体毒性的分子基础在很大程度上仍未被描述.
研究的目的:
- 为了确定涉及到大米病原发生的Ustilaginoidea virens的关键毒性因素.
- 阐明Ustilaginoidea virens抑制大米免疫力的分子机制.
主要方法:
- 鉴定和表征一种分泌的Ustilaginoidea virens蛋白,UV1809,作为一种毒性因子.
- 通过使用异质表达和宿主诱导的基因沉默,研究Uv1809在米敏感性中的作用.
- 分析Uv1809对大米基因组脱乙酶OsSRT2和表观遗传修饰 (H4K5ac,H4K8ac) 的影响.
- 使用CRISPR-Cas9基因编辑来创建和评估ossrt2突变体的疾病抵抗力.
主要成果:
- Uv1809被确定为一种关键的毒性因子,增加了对米假污染和细菌病菌的易感性.
- 在大米中沉默Uv1809显著增加了对Ustilaginoidea virens感染的抵抗力.
- Uv1809通过促进OSSRT2-介导的组分素脱乙烯化来抑制大米免疫力,减少防御基因的激活.
- 克里斯普尔-Cas9产生的ossrt2突变体表现出广泛的抗病能力,而不会影响生长或产量.
结论:
- Uv1809是Ustilaginoidea virens使用的关键效应分子,可以在表观遗传水平上抑制宿主免疫力.
- OsSRT2在防治大米方面发挥着至关重要的作用,其调制为培育抗病大米品种提供了有前途的途径.
- 了解植物病原体相互作用中的表观遗传调节为开发可持续的疾病管理策略提供了新的见解.
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