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Feng Guo¹, Yang Luo¹, GuangYao Ye¹

¹Department of Gastrointestinal Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai City, 200127, China.

Acta biochimica Polonica

|September 16, 2023

概括

循环METTL15 (circMETTL15) 促进结肠直肠癌 (CRC) 的进展,通过海绵miR-374a-5p调节ESCO2. 抑制circMETTL15可能为CRC患者提供一种新的治疗策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

结肠直肠癌 (CRC) 仍然是一个重大的全球健康挑战.
了解驱动CRC进展的分子机制对于开发有效疗法至关重要.

研究的目的:

研究circMETTL15在结肠直肠癌中的生物学作用和潜在的分子机制.
探索circMETTL15作为CRC的治疗点的潜力.

主要方法:

使用定量实时PCR (RT-qPCR) 和西部血栓检测用于检测基因和蛋白质表达水平.
损失和功能增益测试,救援实验,CCK-8,殖民地形成,流细胞计和Transwell测试进行了评估细胞行为.
生物信息学分析和验证试验被用来阐明circMETTL15,miR-374a-5p和ESCO2.p之间的目标关系.

主要成果:

发现CircMETTL15在CRC组织和细胞中高度表达,与晚期瘤阶段和转移相关.
抑制circMETTL15抑制了CRC细胞的增殖,迁移,入侵和上皮-介质细胞过渡 (EMT),同时诱导了细胞亡.
CircMETTL15作为对 miR-374a-5p 的竞争性内源RNA (ceRNA) 起作用,从而调节ESCO2表达并促进CRC进展.

结论:

在通过circMETTL15/miR-374a-5p/ESCO2轴促进结直肠癌进展方面,CircMETTL15发挥着至关重要的作用.
CircMETTL15代表了结直肠癌的潜在诊断生物标志物和治疗点.

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