马雷克病病毒产生的瘤微环境抑制了产生干扰素的玛-玛三角形T细胞
Ayumi Matsuyama-Kato1, Nitish Boodhoo1, Sugandha Raj1
1Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
Veterinary microbiology
|September 16, 2023
概括
马雷克病病毒 (MDV) 感染中的瘤微环境 (TME) 抑制组织内居住的马三角 (γδ) T 细胞. 循环中的γδ T细胞不受这种免疫抑制TME的影响.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 在瘤学瘤学.
背景情况:
- 瘤微环境 (TME) 涉及瘤,免疫和树皮细胞之间的复杂相互作用.
- 马雷克病病毒 (MDV) 感染会产生免疫抑制性TME,可能涉及免疫抑制分子和调节性T细胞.
- 玛三角 (γδ) T细胞在人类癌症中具有抗瘤和前瘤作用,存在于感染MDV的中,但它们的TME相互作用尚不清楚.
研究的目的:
- 调查MDV诱导的TME对循环和组织居住的γδ T细胞的影响.
- 为了确定感染的MDV转化细胞或细胞是否会影响γδ T细胞功能.
主要方法:
- 从患有MDV的培养脊髓细胞和MDV转型的细胞系.
- 通过T细胞受体 (TCR) 刺激刺激组织居民和循环的γδ T细胞.
- 评估培养上游剂对γδ T细胞激活,干扰素 (IFN) -γ产生和CD25表达的影响.
主要成果:
- 来自MDV挑战的脊髓细胞的超活体抑制了TCRγδ刺激的组织居民γδ T细胞中IFN-γ的产生和CD25的表达.
- 来自MDV转化细胞系的超剂没有影响γδ T细胞激活.
- 这两种超剂都没有影响TCRγδ刺激的循环的γδ T细胞的激活或IFN-γ的产生.
结论:
- 由MDV产生的TME可以抑制组织内居住的γδ T细胞的激活和功能.
- 受到瘤吸引的循环的γδ T细胞可能不会受到MDV诱导的免疫抑制TME的类似影响.
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