核因子kappa B在非小细胞肺癌中的表达
Leilei Zhang1, Claudia M Ludden2, Alexander J Cullen2
1Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC, USA.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
|September 16, 2023
概括
核因子-卡帕B (NF-κB) 是一个关键的炎症因子,促进非小细胞肺癌 (NSCLC) 的进展. 它的活性由S-谷氨基化调节,影响患者的生存率.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 生物化学 生物化学
背景情况:
- 核因子-kappa B (NF-κB) 是一种促炎转录因子,调节炎症,增殖和亡中的基因.
- NF-κB失调与各种人类癌症有关,包括非小细胞肺癌 (NSCLC).
- 翻译后的S-glutathionylation通过与谷氨 (GSH) 的二硫化键形成,可逆地改变NF-κB活性.
研究的目的:
- 审查NF-κB在癌症,特别是NSCLC中的作用.
- 调查S-glutathionylation对NF-κB活性的影响及其在NSCLC中的临床相关性.
主要方法:
- 对NF-κB功能和调节的现有文献的审查.
- 对临床数据分析,将NF-κB表达与NSCLC患者预后相关联.
- 检查S-Glutathionylation对NF-κBDNA结合和细胞反应的影响.
主要成果:
- 高NF-κB表达与NSCLC患者的总生存期 (OS) 较短相关,这表明它有促进瘤的作用.
- NF-κB水平与瘤阶段,淋巴结转移和减少5年生存期有关.
- 通过抑制其DNA结合活性,S-Glutathionylation对NF-κB进行负面调节,并与增强的肺炎有关.
结论:
- NF-κB在NSCLC的进展中起着重要作用,并与不良的预后有关.
- S-Glutathionylation是NF-κB的一个关键调节机制.
- 通过S-glutathionylation了解NF-κB调节可能为NSCLC提供治疗策略.
更多相关视频
10:57NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
Published on: January 12, 2020
10.6K
08:26A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry
Published on: April 9, 2013
17.9K
相关概念视频
02:26NF-κB-dependent Signaling Pathway
7.5K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.5K
01:45Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
02:04Co-activators and Co-repressors
7.4K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.4K
01:46Nuclear Localization Signals and Import
5.8K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of 2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
5.8K
02:55The Nucleolus
8.9K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.9K
02:35Chromatin Position Affects Gene Expression
23.4K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
23.4K