多糖类对蛋白质-多二元复合物的结构变化和功能性质的影响:一项比较研究
Zifan Zhang1, Taoran Li1, Yubo Zhang1
1College of Public Health and Health Sciences, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
International journal of biological macromolecules
|September 16, 2023
概括
用多糖化合物研究了大豆蛋白分离物 (SPI) - 奎尔丁 (Que) 复合物. 甲基酸盐 (SA) 改善了复合体的稳定性和功能性质,而德克斯 (DX) 的效果较小.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生化学
- 材料科学 材料科学 材料科学
背景情况:
- 大豆蛋白分离物 (SPI) 是一种有价值的食品成分.
- 奎尔 (Que) 是一种具有抗氧化特性的生物活性黄类化合物.
- 多糖类可以改变蛋白质-碳水化合物复合物的特性.
研究的目的:
- 调查藻酸盐 (SA) 和 (DX) 如何影响SPI-Que复合物的结合和特性.
- 了解SPI,Que和不同多糖之间的相互作用.
- 评估对乳化,发泡和抗氧化活性等功能性质的影响.
主要方法:
- 多光谱技术和斯特恩-沃尔默方程用于研究结合.
- 对结构变化进行胺带 (FTIR) 分析.
- 循环二重化 (CD) 光谱测量以评估二次结构.
- 分子对接和动态模拟以建模相互作用.
主要成果:
- 静态光火证实了Que和SPI的结合.
- SA降低了SPI-Que的结合亲和力,而DX显示出促进作用.
- 多糖添加增加了SPI中的随机卷轴结构.
- SA 增强了 SPI-Que 复合物的稳定性和刚性,而不是 DX.
- SA复合物显示出改善的乳化,发泡和抗氧化特性.
结论:
- 酸显著改变了SPI-Que复合物的结构和稳定性.
- 这些相互作用是由静电力 (SPI-SA) 和范德瓦尔斯力 (SPI-DX) 驱动的.
- 添加SA导致SPI-Que复合物的功能性质得到改善,这表明其在食品配方中的潜在应用.
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