DKK3表达与头部和部状细胞癌的预后较差相关:基于TCGA数据库的生物信息学研究
Naoki Katase1, Shin-Ichiro Nishimatsu2, Akira Yamauchi3
1Department of Oral Pathology, Graduate School of Biomedical Sciences, Nagasaki University, Sakamoto 1-7-1, Nagasaki, Nagasaki, 852-8588, Japan.
Journal of oral biosciences
|September 16, 2023
概括
Dickkopf WNT信号通路抑制剂3 (DKK3) 促进了头部和部状细胞癌 (HNSCC) 的进展. 高DKK3表达与较差的HNSCC预后有关,并呈现出潜在的治疗标.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 之前的研究表明,迪克科普的WNT信号通路抑制剂3 (DKK3) 表达与头部和部状细胞癌 (HNSCC) 的预后不佳相关.
- DKK3在HNSCC进展中的作用需要进一步调查.
研究的目的:
- 使用癌症基因组图谱 (TCGA) 数据库调查HNSCC中的DKK3表达模式.
- 分析DKK3表达和临床数据之间的相关性,确定差异表达基因 (DEGs),并探索相关的生物途径.
主要方法:
- 利用TCGARNA序列数据,将样品分为"DKK3-高"和"DKK3-低"组.
- 使用DESEq2进行DEG识别,使用ShinyGO进行丰富分析,使用GSEA进行途径分析,使用TargetMine进行蛋白与蛋白相互作用 (PPI) 网络构建.
主要成果:
- 在HNSCC样本中,DKK3表达显著升高,高DKK3与较短的整体存活率相关.
- 确定了854个DEGs (284个上调,570个下调).
- 功能丰富突出了与细胞外矩阵重塑相关的途径;PPI网络中的关键基因包括COL8A1,AGTR1,FN1,P4HA3,PDGFRB和CEP126.
结论:
- DKK3在HNSCC中表现出促进癌症的特性.
- DKK3表达作为HNSCC的潜在预后生物标志物.
- DKK3代表了HNSCC治疗的一个有前途的治疗标.
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