Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Advances in diagnostic methods for detection of bloodstream pathogens and antibiotic resistance determinants.

Frontiers in cellular and infection microbiology·2025
Same author

Multiplexed suspension array immunoassays for detection of antibodies to pneumococcal polysaccharide and conjugate vaccines.

Frontiers in cellular and infection microbiology·2023
Same author

Diagnosis and Management of Bloodstream Infections With Rapid, Multiplexed Molecular Assays.

Frontiers in cellular and infection microbiology·2022
Same author

Comparison of xMAP <i>Salmonella</i> Serotyping Assay With Traditional Serotyping and Discordance Resolution by Whole Genome Sequencing.

Frontiers in cellular and infection microbiology·2020
Same author

Evaluation of the Aries <i>Bordetella</i> Assay for Detection and Identification of <i>Bordetella pertussis</i> in Nasopharyngeal Swab Specimens.

Journal of clinical microbiology·2019
Same author

The genesis and evolution of bead-based multiplexing.

Methods (San Diego, Calif.)·2019

相关实验视频

Updated: Jul 16, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

10.4K

基于珠子的悬浮阵列的核酸样品制备技术

Sherry A Dunbar1

  • 1Scientific Affairs, Luminex, A DiaSorin Company, 12212 Technology Blvd., Austin, TX 78727, USA.

Methods (San Diego, Calif.)
|September 16, 2023
PubMed
概括

多重复合试验可同时检测多种分析物,降低成本和时间. 本综述强调了核酸提取和净化方法,这些方法对于生物分析中的基于珠子的分析至关重要.

科学领域:

  • 生物技术和分子生物学
  • 测试开发 测试开发
  • 基因组学就是基因组学.

背景情况:

  • 多重复合测试可有效地同时检测多种分析物,与单个分析物方法相比,可减少时间,劳动力和成本.
  • 基于珠子的悬浮阵列技术,如Luminex xMAP,使用各种测试化学方法促进高通量核酸检测.
  • 高效的核酸提取和净化是基于珠子的微阵列平台和其他核酸化学的关键先决条件.

结论:

  • 优化核酸提取和净化对于基于珠子的多重测试中可靠和准确的结果至关重要.
  • 了解样本特定的挑战和测试要求是选择适当净化策略的关键.
  • 本综述为使用基于珠子的平台进行核酸分析的研究人员提供了有价值的见解.
关键词:
基于珠子的珠子制品.这就是为什么Luminex.多重复合体是一个多重复合体.核酸是核酸中的一种.悬架阵列是一个悬架阵列.在 xMAPAP 里面.

更多相关视频

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
08:26

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

13.7K
Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
09:19

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

Published on: March 26, 2018

9.1K

相关实验视频

Last Updated: Jul 16, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

10.4K
Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
08:26

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

13.7K
Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
09:19

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

Published on: March 26, 2018

9.1K